Related Experiment Video
Updated: May 10, 2026

Rapid Identification of Gram Negative Bacteria from Blood Culture Broth Using MALDI-TOF Mass Spectrometry
Published on: May 28, 2014
[Direct identification method for bacteria in positive blood culture bottles using MALDI biotyper]
Nozomi Uki1, Megumi Oho, Zenzo Nagasawa
1Department of Clinical Laboratory, Saga University Hospital, Saga 849-8501, Japan. ukinozo@cc.saga-u.ac.jp
Abstract:
We investigated the relevance of direct identification method for bacteria from blood culture bottles using MALDI Biotyper(Bruker Co.). One hundred fourteen bacterial strains obtained from blood culture bottles were analyzed using the pretreatment method recommended by Bruker Corporation. The identification rate with recommended pretreatment was 76.3% (87/114). Twenty seven samples that were not identified with the pretreatment method were further analyzed using our modified method. Of these, twelve were identified, improving the identification rate to 86.8% (99/114). These results suggest that our modified pretreatment combined with pretreatment method recommended by Bruker Corporation yields improved identification of bacteria from blood culture bottles using MALDI Biotyper system.
More Related Videos
09:07Direct Microbial Identification using An Automated Microbial Identification System to Facilitate the EUCAST RAST Method Without Mass Spectrometry
Published on: May 24, 2024
11:25Preparation of a Blood Culture Pellet for Rapid Bacterial Identification and Antibiotic Susceptibility Testing
Published on: October 15, 2014
Related Concept Videos
Methods of Classification and Identification
Automated Microbial Diagnostics
Differential Staining Technique
MALDI-TOF Mass Spectrometry
Modern Molecular Taxonomy