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Updated: May 10, 2026

Conducting Multiple Imaging Modes with One Fluorescence Microscope
Published on: October 28, 2018
3-D stimulated emission depletion microscopy with programmable aberration correction
Martin O Lenz1, Hugo G Sinclair, Alexander Savell
1Photonics Group, Imperial College London, London SW7 2AZ, UK.
Abstract:
We present a stimulated emission depletion (STED) microscope that provides 3-D super resolution by simultaneous depletion using beams with both a helical phase profile for enhanced lateral resolution and an annular phase profile to enhance axial resolution. The 3-D depletion point spread function is realised using a single spatial light modulator that can also be programmed to compensate for aberrations in the microscope and the sample. We apply it to demonstrate the first 3-D super-resolved imaging of an immunological synapse between a Natural Killer cell and its target cell.
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