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Determination of malondialdehyde with chemical derivatization into the pyrimidine compound and HPLC
E Kishida1, M Oribe, K Mochizuki
1Department of Life and Health Sciences, Hyogo University of Teacher Education, Japan.
Biochimica Et Biophysica Acta
|July 16, 1990
Abstract:
A new HPLC method to determine malondialdehyde (MDA) was developed. Malondialdehyde was reacted with alpha-N-benzoyl-L-arginine ethyl ester and converted into alpha-N-benzoyl-delta-N-(2-pyrimidinyl)-L-ornithine ethyl ester, which is sufficiently hydrophobic to allow us its specific determination utilizing a reversed-phase C18 column at the level of 1 pmol.