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Standardized Method for High-throughput Sterilization of Arabidopsis Seeds
Published on: October 17, 2017
Massive genomic variation and strong selection in Arabidopsis thaliana lines from Sweden
Quan Long1, Fernando A Rabanal1, Dazhe Meng2
1Gregor Mendel Institute, Austrian Academy of Sciences, Vienna, Austria.
Abstract:
Despite advances in sequencing, the goal of obtaining a comprehensive view of genetic variation in populations is still far from reached. We sequenced 180 lines of A. thaliana from Sweden to obtain as complete a picture as possible of variation in a single region. Whereas simple polymorphisms in the unique portion of the genome are readily identified, other polymorphisms are not. The massive variation in genome size identified by flow cytometry seems largely to be due to 45S rDNA copy number variation, with lines from northern Sweden having particularly large numbers of copies. Strong selection is evident in the form of long-range linkage disequilibrium (LD), as well as in LD between nearby compensatory mutations. Many footprints of selective sweeps were found in lines from northern Sweden, and a massive global sweep was shown to have involved a 700-kb transposition.
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