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Updated: May 10, 2026

Nuclear Magnetic Resonance Spectroscopy for the Identification of Multiple Phosphorylations of Intrinsically Disordered Proteins
Published on: December 27, 2016
Systematic assignment of NMR spectra of 5-substituted-4-thiopyrimidine nucleosides
Xiaohui Zhang1, Jian Wang, Yao-Zhong Xu
1College of Environment and Chemical Engineering, Dalian University, Dalian 116622, China. zhangxiaohui@dlu.edu.cn
Abstract:
Unambiguous characterization of 5-substituted-4-thiopyrimidine nucleosides (ribonucleosides and 2'-deoxynucleosides) was performed using NMR spectroscopy. Assignments of all proton and carbon signals of 5-bromo-4-thiouridine and related nucleosides were systematically carried out and firmly established by COSY and HMQC techniques. The NMR data of various 4-thiopyrimidine nucleosides are compared, and the key contributing factors discussed. The approach presented here is applicable to other modified nucleosides and nucleotides, as well as nucleobases.
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