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Related Concept Videos

Fixation and Sectioning01:03

Fixation and Sectioning

Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens.
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...
DNA Agarose Gel Electrophoresis02:35

DNA Agarose Gel Electrophoresis

Agarose gel electrophoresis is a laboratory technique commonly used to separate DNA fragments by size. However, it can also be used to isolate and purify DNA fragments using a gel extraction protocol.
Gel extraction follows five major steps: running gel electrophoresis to separate fragments, isolating the individual bands, extracting DNA from those bands, and removing the dye and salts from the extracted mixture to obtain pure DNA.
In cloning experiments, both the insert and vector DNA...
Special Staining Techniques01:13

Special Staining Techniques

Specialized staining techniques play a vital role in microbiology by enabling the visualization of specific bacterial structures that remain undetectable with standard microscopy methods. These techniques not only enhance the structural visualization of bacterial cells but also provide critical insights into their pathogenicity and classification. Additionally, they support diagnostic and research endeavors in microbiology by identifying key bacterial features.Capsule Staining for Virulence...
Differential Staining Technique01:26

Differential Staining Technique

Differential staining is an essential microbiological technique that exploits variations in cell wall structures to classify and identify microorganisms. It facilitates the distinction of bacteria, aiding in diagnostic and research applications. Two of the most widely used differential staining methods are Gram staining and acid-fast staining, both of which rely on the chemical and structural differences in bacterial cell walls.Gram Staining TechniqueGram staining differentiates bacteria by...
Simple Staining Technique01:24

Simple Staining Technique

OverviewStaining techniques in microscopy enhance the visualization of microorganisms by increasing contrast and allowing the differentiation of cellular structures. Simple staining is one of the fundamental methods used to observe the basic morphological characteristics of microorganisms, including their size, shape, and arrangement. This method relies on the application of a single dye to stain the entire cell, producing a clear contrast between the cell and the background.FixationFixation is...
FISH - Fluorescent In-situ Hybridization02:07

FISH - Fluorescent In-situ Hybridization

Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...

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Related Experiment Video

Updated: May 10, 2026

DNA Staining Method Based on Formazan Precipitation Induced by Blue Light Exposure
08:11

DNA Staining Method Based on Formazan Precipitation Induced by Blue Light Exposure

Published on: January 28, 2018

A method for sensitive staining of DNA in polyacrylamide gels using basic fuchsin.

Mao Chen1, Wei-Tao Cong, Xuan Zhou

  • 1Zhejiang Provincial Key Laboratory of Biopharmaceuticals, Wenzhou Medical College; Wenzhou, Zhejiang, PR China.

Bioanalysis
|June 26, 2013
PubMed
Summary

Basic fuchsin (BF) offers a fast and sensitive visible dye stain for DNA in polyacrylamide gels. This method detects as little as 10-20 pg of DNA within 10 minutes, outperforming fluorescent stains.

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Last Updated: May 10, 2026

DNA Staining Method Based on Formazan Precipitation Induced by Blue Light Exposure
08:11

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Published on: January 28, 2018

Simple Method for Fluorescence DNA In Situ Hybridization to Squashed Chromosomes
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Fluorescent Silver Staining of Proteins in Polyacrylamide Gels
06:24

Fluorescent Silver Staining of Proteins in Polyacrylamide Gels

Published on: April 21, 2019

Area of Science:

  • Molecular Biology
  • Biochemistry
  • Analytical Chemistry

Background:

  • Polyacrylamide gel electrophoresis (PAGE) is a standard technique for DNA mixture separation by size.
  • Current DNA visualization methods (fluorescence, visible dye, silver staining) have varying sensitivity, safety, and simplicity.
  • There is a need for improved DNA detection methods in PAGE.

Purpose of the Study:

  • To develop and characterize a novel, sensitive visible dye-based staining method for DNA in polyacrylamide gels.
  • To evaluate the sensitivity and speed of the new staining method compared to existing techniques.

Main Methods:

  • Development of a basic fuchsin (BF) staining protocol for DNA in polyacrylamide gels.
  • Sensitivity testing using varying amounts of DNA.
  • Comparison of BF staining with SYBR® Gold and silver staining.
  • Mechanistic studies to understand the DNA-BF interaction.

Main Results:

  • A fast (10 min) and sensitive visible dye-based staining method using basic fuchsin (BF) was established.
  • The method can visualize as low as 10-20 pg of DNA.
  • BF staining sensitivity is fourfold higher than SYBR® Gold and comparable to silver staining.
  • Mechanism studies indicate a non-intercalative binding mode between BF and DNA.

Conclusions:

  • Basic fuchsin (BF) provides a rapid and highly sensitive visible dye-based staining method for DNA detection in polyacrylamide gels.
  • This protocol offers a viable alternative to existing DNA visualization techniques.