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Updated: May 10, 2026

Enzymatic Cascade Reactions for the Synthesis of Chiral Amino Alcohols from L-lysine
Published on: February 16, 2018
Synthesis of enantiopure fluorohydrins using alcohol dehydrogenases at high substrate concentrations
Wioleta Borzęcka1, Iván Lavandera, Vicente Gotor
1Departamento de Química Orgánica e Inorgánica, University of Oviedo, C/Julián Clavería 8, 33006 Oviedo, Spain.
Abstract:
The use of purified and overexpressed alcohol dehydrogenases to synthesize enantiopure fluorinated alcohols is shown. When the bioreductions were performed with ADH-A from Rhodococcus ruber overexpressed in E. coli, no external cofactor was necessary to obtain the enantiopure (R)-derivatives. Employing Lactobacillus brevis ADH, it was possible to achieve the synthesis of enantiopure (S)-fluorohydrins at a 0.5 M substrate concentration. Furthermore, due to the activated character of these substrates, a huge excess of the hydrogen donor was not necessary.
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