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Preliminary Study to Assess the Performance of Mengovirus Elution from Sludge
Hasna Amdiouni1, Abdelaziz Soukri, Jalal Nourlil
1Medical Virology & BSL3 Laboratory, Institut Pasteur du Maroc. 1, Place Louis Pasteur, 20360, Casablanca, Morocco, hasna.amdiouni@yahoo.fr.
Abstract:
In the virus detection protocol for sludge, the viral elution step from solids to solution is critical. In this study, mengoviruses were detected in artificially contaminated sludge with a qRT-PCR assay. The viral yields ranged between 19 and 66 % for 60 % sludge. This study demonstrates that mengovirus can be used as a sample process control for analysis of sewage sludge.
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