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Related Concept Videos

Immunogold Electron Microscopy01:20

Immunogold Electron Microscopy

Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.
Immunocytochemistry and Immunohistochemistry01:22

Immunocytochemistry and Immunohistochemistry

Immunocytochemistry (ICC) and immunohistochemistry (IHC) are techniques that use antibodies to check for specific proteins or antigens in a sample. The technique was first published by Albert Coons in 1941 to detect the presence of pneumococcal antigen in tissue sections from mice infected with Pneumococcus. Immunocytochemistry helps localization of proteins or antigens in individual cells like blood cells, stem cells, etc., while immunohistochemistry does the same for tissue samples.
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Related Experiment Video

Updated: May 10, 2026

Post-embedding Immunogold Labeling of Synaptic Proteins in Hippocampal Slice Cultures
13:50

Post-embedding Immunogold Labeling of Synaptic Proteins in Hippocampal Slice Cultures

Published on: April 3, 2013

Immunogold cytochemistry in neuroscience.

Mahmood Amiry-Moghaddam1, Ole Petter Ottersen

  • 1Laboratory of Molecular Neuroscience, Institute of Basic Medical Sciences, University of Oslo, Oslo, Norway.

Nature Neuroscience
|June 27, 2013
PubMed
Summary

Immunogold procedures offer precise protein localization in the complex central nervous system. While widely used in neuroscience, understanding their limitations and maximizing their potential is crucial for advanced research.

Area of Science:

  • Neuroscience
  • Cell Biology
  • Biochemistry

Background:

  • The central nervous system's complexity necessitates advanced techniques for protein localization.
  • Immunocytochemical methods are vital for precise target protein identification and quantification.

Purpose of the Study:

  • To highlight the power of immunogold procedures for high-precision protein localization in the central nervous system.
  • To discuss the limitations and potential applications of immunogold techniques in neuroscience.

Main Methods:

  • Review and analysis of immunogold procedures in neuroscience research.
  • Discussion of technical challenges and optimization strategies for immunogold labeling.

Main Results:

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Immunostaining of Biocytin-filled and Processed Sections for Neurochemical Markers
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Immunostaining of Biocytin-filled and Processed Sections for Neurochemical Markers

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High-Resolution Quantitative Immunogold Analysis of Membrane Receptors at Retinal Ribbon Synapses
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High-Resolution Quantitative Immunogold Analysis of Membrane Receptors at Retinal Ribbon Synapses

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Last Updated: May 10, 2026

Post-embedding Immunogold Labeling of Synaptic Proteins in Hippocampal Slice Cultures
13:50

Post-embedding Immunogold Labeling of Synaptic Proteins in Hippocampal Slice Cultures

Published on: April 3, 2013

Immunostaining of Biocytin-filled and Processed Sections for Neurochemical Markers
08:54

Immunostaining of Biocytin-filled and Processed Sections for Neurochemical Markers

Published on: December 31, 2016

High-Resolution Quantitative Immunogold Analysis of Membrane Receptors at Retinal Ribbon Synapses
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High-Resolution Quantitative Immunogold Analysis of Membrane Receptors at Retinal Ribbon Synapses

Published on: February 18, 2016

  • Immunogold procedures provide exceptional spatial resolution for protein mapping.
  • Identified common pitfalls include background noise and antibody penetration issues.
  • Highlighted opportunities for improved quantitation and multiplexed labeling.

    • Conclusions:

    • Immunogold techniques are indispensable tools for neuroscientists.
    • Further research is needed to fully exploit the potential of these powerful methods.
    • Addressing current limitations will enhance the reliability and scope of immunogold applications.