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Acidification in the rat proximal jejunum.

J A Blair, M L Lucas, A J Matty

    The Journal of Physiology
    |February 1, 1975
    PubMed
    Summary
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    Rat jejunum produces hydrogen ions, with glucose enhancing this process. ATP breakdown at the mucosal surface is proposed as the primary mechanism for this acidification.

    Area of Science:

    • Gastroenterology
    • Cell Physiology
    • Biochemistry

    Background:

    • The proximal jejunum's role in ion transport is crucial for nutrient absorption and maintaining intestinal homeostasis.
    • Understanding the mechanisms of hydrogen ion production in the jejunum is essential for comprehending digestive physiology.

    Purpose of the Study:

    • To investigate the production of hydrogen ions by the rat proximal jejunum.
    • To elucidate the metabolic pathways and regulatory factors involved in jejunal acidification.

    Main Methods:

    • Utilized the everted sac technique to study hydrogen ion production in rat jejunum.
    • Assessed the effects of glucose, various inhibitors (2,4-dinitrophenol, phlorrhizin, aminophylline), and different hexoses (galactose, mannose, fructose) on acidification.

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  • Measured lactate production and evaluated the impact of Adenosine Triphosphate (ATP) and other compounds.

    • Main Results:

    • Hydrogen ion production was observed, significantly enhanced by glucose (apparent Km of 1.78 mM).
    • Acidification was inhibited by 2,4-dinitrophenol, phlorrhizin, aminophylline, and anaerobiosis, indicating a metabolic dependence.
    • Mannose and fructose increased acidification, suggesting a metabolic origin of hydrogen ions, while galactose and 3-O-methylglucose did not.
    • Lactate production accounted for only a small fraction of hydrogen ions; ATP administration increased acidification without significant lactate production.

    Conclusions:

    • Jejunal acidification is a glucose-dependent metabolic process.
    • The findings support a hypothesis where intracellular ATP breakdown at the mucosal surface contributes to hydrogen ion production and the intestinal microclimate.