Related Experiment Video
Updated: May 10, 2026

A Flame-Free Method for Sterilizing C. elegans Picks, Spatulas, and Scalpels
Published on: March 4, 2022
Immediate use steam sterilization: it's all about the process
1Portage Health, Hancock, MI, USA.
Abstract:
Proper reprocessing of surgical instrumentation assists in infection prevention efforts. Immediate use steam sterilization, previously known as flash sterilization, is an effective method of sterilization if it is performed according to manufacturers' validated instructions. Items that undergo immediate use steam sterilization are subject to the same cleaning and decontamination requirements that apply to preparing items for terminal sterilization, and manufacturers' instructions for use must be strictly followed. In 2009, The Joint Commission revised its position statement on flash sterilization and emphasized that three critical steps of reprocessing must be followed to ensure sterility (ie, cleaning and decontamination, sterilization, aseptic transfer) and that complete documentation must be available for each immediate use steam sterilization cycle so that the device is traceable to the patient if problems arise.
Related Concept Videos
Methods of Sterilization I: Physical Methods
Steam sterilization uses non-toxic, low-cost moist heat in the form of saturated steam under pressure, which is fast, microbicidal, and sporicidal, and quickly warms and penetrates fabrics. Autoclaves, or steam sterilizers, expose each item to direct steam contact for a predetermined time at the necessary...
Methods of Sterilization II: Chemical Methods
Using chemical sterilization rather than heat to clean out equipment is recommended. It eradicates and removes all bacteria,...
Cleaning, Sterilization, and Disinfection
Cleaning
The cleaning process usually involves using water with detergents or enzymatic cleaner and removing foreign material from objects and surfaces, including organic material such as body fluids or inorganic material like soil. Cleaning is performed before high-level disinfection and sterilization because foreign materials on the cover of the devices interfere with process...
Physical Methods for Controlling Microbial Growth: Temperature
Physical Methods for Controlling Microbial Growth: Radiation and Filtration
Scale-Up Processes

