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Related Experiment Video

Updated: May 10, 2026

Fabrication and Operation of Acoustofluidic Devices Supporting Bulk Acoustic Standing Waves for Sheathless Focusing of Particles
10:14

Fabrication and Operation of Acoustofluidic Devices Supporting Bulk Acoustic Standing Waves for Sheathless Focusing of Particles

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Elastomeric microparticles for acoustic mediated bioseparations.

Leah M Johnson1, Lu Gao, C Wyatt Shields IV

  • 1Department of Biomedical Engineering, Duke University, 101 Science Drive, 3361 CIEMAS, Durham, NC 27708, USA.

Journal of Nanobiotechnology
|July 2, 2013
PubMed
Summary
This summary is machine-generated.

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This study introduces a novel acoustophoretic cell sorting method using biofunctionalized particles to specifically capture and transport target cells, overcoming limitations of traditional physical property-based sorting.

Area of Science:

  • Biophysics
  • Acoustofluidics
  • Bioseparation

Background:

  • Acoustophoresis is limited by reliance on inherent cell physical properties for sorting.
  • Biochemical specificity is needed to advance acoustophoresis for cell sorting in research and clinical settings.

Purpose of the Study:

  • To develop an acoustophoretic cell sorting approach with biochemical specificity.
  • To demonstrate the use of biofunctionalized particles for targeted cell transport in ultrasound standing waves.

Main Methods:

  • Fabrication of stable negative acoustic contrast particles (NACPs) using polydimethylsiloxane (PDMS) or polyvinylmethylsiloxane (PVMS).
  • Biofunctionalization of NACPs via photochemical reactions or surfactant end-functionalization.
  • Utilizing biotinylated NACPs to capture and transport streptavidin-coated microparticles (cell surrogates) in an acoustofluidic chip.

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Published on: November 23, 2015

Microparticle Manipulation by Standing Surface Acoustic Waves with Dual-frequency Excitations
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Microparticle Manipulation by Standing Surface Acoustic Waves with Dual-frequency Excitations

Published on: August 21, 2018

Related Experiment Videos

Last Updated: May 10, 2026

Fabrication and Operation of Acoustofluidic Devices Supporting Bulk Acoustic Standing Waves for Sheathless Focusing of Particles
10:14

Fabrication and Operation of Acoustofluidic Devices Supporting Bulk Acoustic Standing Waves for Sheathless Focusing of Particles

Published on: March 6, 2016

A Microfluidic Platform for Precision Small-volume Sample Processing and Its Use to Size Separate Biological Particles with an Acoustic Microdevice
11:32

A Microfluidic Platform for Precision Small-volume Sample Processing and Its Use to Size Separate Biological Particles with an Acoustic Microdevice

Published on: November 23, 2015

Microparticle Manipulation by Standing Surface Acoustic Waves with Dual-frequency Excitations
06:51

Microparticle Manipulation by Standing Surface Acoustic Waves with Dual-frequency Excitations

Published on: August 21, 2018

Main Results:

  • Demonstrated successful capture and transport of positive acoustic contrast particles (PACPs) by NACPs to the antinode of an ultrasound standing wave.
  • Showcased biofunctionalization of NACPs for specific binding to target microparticles.
  • Confirmed transport of biotinylated NACPs with bound streptavidin microparticles to the pressure antinode.

Conclusions:

  • First demonstration of NACPs as carriers for PACP transport in ultrasound standing waves.
  • Highlighted the versatility of silicone materials and curing chemistries for NACP preparation.
  • Proposed this bioseparation scheme for rapid, continuous cell and biomolecule sorting and analysis.