Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Racemic Mixtures and the Resolution of Enantiomers02:30

Racemic Mixtures and the Resolution of Enantiomers

A racemic mixture, or racemate, is an equimolar mixture of enantiomers of a molecule that can be separated using their unique interaction with chiral molecules or media. Racemic mixtures are denoted by the (±)- prefix. This ‘optical rotation descriptor’ applies to the whole solution of a racemic mixture rather than a specific stereoisomer. Enantiomers typically have the same physical and chemical properties. Hence, they are not easily separable. However, enantiomers can exhibit different...
Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
¹H NMR: Complex Splitting01:13

¹H NMR: Complex Splitting

A proton M that is coupled to a proton X results in doublet signals for M. However, NMR-active nuclei can be simultaneously coupled to more than one nonequivalent nucleus. When M is coupled to a second proton A, such as in styrene oxide, each peak in the doublet is split into another doublet.
Splitting diagrams or splitting tree diagrams are routinely used to depict such complex couplings. While drawing splitting diagrams, the splitting with the larger coupling constant is usually applied first.
Chromatographic Resolution01:15

Chromatographic Resolution

In chromatography, a solute moves through a chromatographic column and tends to spread, forming a Gaussian-shaped band. The longer the solute spends in the column, the broader the band becomes. The broadening can lead to overlaps within the column, affecting separation effectiveness.
The effectiveness of separation can be evaluated by determining the level of separation between two neighboring peaks in a chromatogram, which represents the individual components of a sample.
In chromatography,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Robust single-scan ultraselective NMR.

Chemical communications (Cambridge, England)·2026
Same author

General Nuclear Magnetic Resonance Analysis Toolbox for Stats: A Comprehensive Module for Nuclear Magnetic Resonance-Based Chemometrics and Metabolomics.

Analytical chemistry·2026
Same author

Practical Guide and Best Practices for Diffusion NMR Processing With GNAT.

Magnetic resonance in chemistry : MRC·2026
Same author

Real-Time NMR Quantification of Paramagnetic Species during Chemical Reactions.

Analytical chemistry·2026
Same author

Full-Signal Ultrahigh-Resolution NMR by Parameter Estimation.

Analytical chemistry·2025
Same author

<sup>1</sup>H NMR Pure Shift Metabolomic Analysis of Black Tea.

Analytical chemistry·2025
Same journal

pyTRACTnmr: an open source python package for analyzing [<sup>15</sup>N, <sup>1</sup>H]-TRACT experiments.

Journal of biomolecular NMR·2026
Same journal

RelCalc: symbolic evaluation of BWR theory relaxation rates in python, applications to TROSY effects in AX[Formula: see text] spin systems.

Journal of biomolecular NMR·2026
Same journal

Solution NMR study of the titin I-band IgI domain I82 shows unusual conformational dynamics.

Journal of biomolecular NMR·2026
Same journal

Methyl-specific NMR of therapeutic antibodies: cost-effective isotopic labeling strategies in CHO cells for high-resolution structural characterization.

Journal of biomolecular NMR·2026
Same journal

AMIGO - Guided assignment of <sup>13</sup>C-methyl labelled proteins.

Journal of biomolecular NMR·2026
Same journal

Super-Resolution solid-state NMR Spectroscopy.

Journal of biomolecular NMR·2026
See all related articles

Related Experiment Video

Updated: May 10, 2026

Controlled Synthesis and Fluorescence Tracking of Highly Uniform Poly(N-isopropylacrylamide) Microgels
11:34

Controlled Synthesis and Fluorescence Tracking of Highly Uniform Poly(N-isopropylacrylamide) Microgels

Published on: September 8, 2016

Resolving complex mixtures: trilinear diffusion data.

Johannes Björnerås1, Adolfo Botana, Gareth A Morris

  • 1Department of Biochemistry and Biophysics, Stockholm University, Svante Arrhenius väg 16C, 106 91, Stockholm, Sweden.

Journal of Biomolecular NMR
|July 2, 2013
PubMed
Summary
This summary is machine-generated.

This study introduces a novel method using multi-dimensional Nuclear Magnetic Resonance (NMR) diffusion data to analyze complex biological mixtures. This technique enhances the characterization of biomacromolecules and metabolites in systems like spider venom and body fluids.

More Related Videos

Synthesis of Cyclic Polymers and Characterization of Their Diffusive Motion in the Melt State at the Single Molecule Level
06:55

Synthesis of Cyclic Polymers and Characterization of Their Diffusive Motion in the Melt State at the Single Molecule Level

Published on: September 26, 2016

Related Experiment Videos

Last Updated: May 10, 2026

Controlled Synthesis and Fluorescence Tracking of Highly Uniform Poly(N-isopropylacrylamide) Microgels
11:34

Controlled Synthesis and Fluorescence Tracking of Highly Uniform Poly(N-isopropylacrylamide) Microgels

Published on: September 8, 2016

Synthesis of Cyclic Polymers and Characterization of Their Diffusive Motion in the Melt State at the Single Molecule Level
06:55

Synthesis of Cyclic Polymers and Characterization of Their Diffusive Motion in the Melt State at the Single Molecule Level

Published on: September 26, 2016

Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Spectroscopy

Background:

  • Biological systems function as complex mixtures, with biomacromolecules and metabolites playing crucial roles.
  • Understanding these mixtures is vital for deciphering biological processes and disease mechanisms.
  • Current methods for mixture characterization are insufficient for complex biological samples.

Purpose of the Study:

  • To develop advanced methods for characterizing complex biological mixtures.
  • To improve the analysis of Nuclear Magnetic Resonance (NMR) spectra from mixtures.
  • To extract detailed information from multi-dimensional NMR diffusion data.

Main Methods:

  • Utilizing multi-dimensional NMR data, specifically extending NMR diffusion data to three or more dimensions.
  • Employing powerful algorithms to decompose and analyze the complex spectral data.
  • Exploiting independently varying data dimensions to disentangle mixture components.

Main Results:

  • A potent method for disentangling NMR spectra of mixture components was developed.
  • The technique allows for the extraction of specific information from complex mixtures.
  • The approach is particularly effective for analyzing NMR diffusion data.

Conclusions:

  • The developed method offers a significant advancement in characterizing complex biological mixtures.
  • This technique can provide deeper insights into biological systems by analyzing metabolite concentrations and biomacromolecule functions.
  • Further application of this method can enhance our understanding of diseases through body fluid analysis.