Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Design, synthesis, and analysis of multi-layered 3D fluorescent polymers derived from anthracene and naphthalene structural units.

RSC advances·2026

Same author

Isolation and detection of target cells in blood <i>via</i> immunomagnetic separation and atomic emission spectroscopy.

Analytical methods : advancing methods and applications·2025

Same author

Evaluating antibiofilm efficacy of carbon dots againstPseudomonas Aeruginosabased on precursor molecule selection.

Colloids and surfaces. B, Biointerfaces·2025

Same author

The Synthesis and Property Study of NH-Ac-Anchored Multilayer 3D Polymers.

Molecules (Basel, Switzerland)·2025

Same author

Effect of precursors on carbon dot functionalization and applications: a review.

The Analyst·2025

Same author

Affinity-based 3D-printed microfluidic chip for clinical sepsis detection with CD69, CD64, and CD25.

Journal of pharmaceutical and biomedical analysis·2024

Same journal

A pump-free gravity-driven microfluidic chip for rapid RPA-LFS-based detection of Magnaporthe oryzae AvrPi9 gene.

Biomedical microdevices·2026

Same journal

Mechanotherapeutic biomaterials: Overcoming physical barriers to enhance intratumoral drug delivery in solid tumours.

Biomedical microdevices·2026

Same journal

Reversibly-sealable microfluidic platform for multi-molecule gradient delivery to large adherent cell cultures.

Biomedical microdevices·2026

Same journal

3D printed chip as platform to vascularize hiPSCs-derived kidney organoids.

Biomedical microdevices·2026

Same journal

Ingestible smart capsules: from engineering innovation to GI drug delivery.

Biomedical microdevices·2026

Same journal

An inexpensive, portable, refrigeration-free, ready-to-use microfluidic device for real-time multiplexed molecular detection of HIV, HBV, and HCV.

Biomedical microdevices·2026

See all related articles

Search research articles

Related Experiment Video

Updated: May 10, 2026

Generation of Heterogeneous Drug Gradients Across Cancer Populations on a Microfluidic Evolution Accelerator for Real-Time Observation

Generation of Heterogeneous Drug Gradients Across Cancer Populations on a Microfluidic Evolution Accelerator for Real-Time Observation

Published on: September 19, 2019

A microfluidic localized, multiple cell culture array using vacuum actuated cell seeding: integrated anticancer drug

Yan Gao¹, Peng Li, Dimitri Pappas

¹Department of Chemistry and Biochemistry, Texas Tech University, Lubbock, TX, 79409, USA.

Biomedical Microdevices

|July 2, 2013

Summary

This summary is machine-generated.

This study presents a novel microfluidic device for culturing multiple cell lines simultaneously. The vacuum-actuated system enables high-purity cell seeding and long-term monitoring for cell-based assays.

More Related Videos

Microfluidic Device for Recreating a Tumor Microenvironment in Vitro

Microfluidic Device for Recreating a Tumor Microenvironment in Vitro

Published on: November 20, 2011

A Microfluidic Platform for High-throughput Single-cell Isolation and Culture

A Microfluidic Platform for High-throughput Single-cell Isolation and Culture

Published on: June 16, 2016

Related Experiment Videos

Last Updated: May 10, 2026

Generation of Heterogeneous Drug Gradients Across Cancer Populations on a Microfluidic Evolution Accelerator for Real-Time Observation

Generation of Heterogeneous Drug Gradients Across Cancer Populations on a Microfluidic Evolution Accelerator for Real-Time Observation

Published on: September 19, 2019

Microfluidic Device for Recreating a Tumor Microenvironment in Vitro

Microfluidic Device for Recreating a Tumor Microenvironment in Vitro

Published on: November 20, 2011

A Microfluidic Platform for High-throughput Single-cell Isolation and Culture

A Microfluidic Platform for High-throughput Single-cell Isolation and Culture

Published on: June 16, 2016

Area of Science:

Biotechnology
Microfluidics
Cell Biology

Background:

Microfluidic devices offer precise control over cellular microenvironments.
Culturing multiple cell lines on a single chip facilitates comparative analysis and reduces resource consumption.

Purpose of the Study:

To develop a novel, user-friendly microfluidic device for simultaneous culture of multiple cell lines.
To demonstrate the device's capability for long-term cell monitoring and cell-based assays.

Main Methods:

A microfluidic device with 8 individually addressable culture chambers was fabricated.
One-step vacuum actuation was employed for high-purity cell line (HuT 78, Ramos, PC-3, C166-GFP) seeding.
Simultaneous on-chip culture and cell viability assays were performed over extended periods (up to 168 hours).
Mass transport via diffusion was studied using fluorescein solution.

Main Results:

High purity (99.9%-100%) seeding of four distinct cell lines was achieved.
Successful simultaneous culture of multiple cell lines for up to 168 hours with high cell viability (>92%).
Demonstrated efficacy in cell-based assays, including apoptosis detection after treatment with staurosporine and anti-human CD95.

Conclusions:

The developed microfluidic device provides an integrated, flexible, and easy-to-use platform for long-term multiple cell culture.
This technology is well-suited for comparative cell-based assays and advanced biological studies.
The device's design facilitates efficient cell monitoring and analysis, paving the way for enhanced research applications.