Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Immunogold Electron Microscopy01:20

Immunogold Electron Microscopy

Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

HEBE: A novel chimeric chronokine for ameliorating memory deficits in Alzheimer's disease.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie·2025
Same author

Roadmap for Postnatal Brain Maturation: Changes in Gray and White Matter Composition during Development Measured by Fourier Transformed Infrared Microspectroscopy.

ACS chemical neuroscience·2023
Same author

Younger age and induction failure predict outcomes in infant leukemia: 30 years of experience in a tertiary center.

Frontiers in pediatrics·2023
Same author

Decoding the molecular heterogeneity of pediatric monomorphic post-solid organ transplant lymphoproliferative disorders.

Blood·2023
Same author

Microglia states and nomenclature: A field at its crossroads.

Neuron·2022
Same author

Tisagenlecleucel therapy for relapsed or refractory B-cell acute lymphoblastic leukaemia in infants and children younger than 3 years of age at screening: an international, multicentre, retrospective cohort study.

The Lancet. Haematology·2022
Same journal

Tracking Synthetic Adhesins on Bacterial Surfaces with Immunofluorescence Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Post-Selection Methods for Analyzing mRNA Display Selections and Optimization of Hits.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

High-Performance Computing in Tandem Mass Spectrometry (MS/MS) Peptide Identification.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Engineering and Adapting Disulfide-Containing Proteins to Enable Intracellular Functionality.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

AI-Driven Protein Research: From Prediction to Design.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Methods for the In Vitro Selection of Protein and Peptide Libraries Using mRNA Display.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

Related Experiment Video

Updated: May 10, 2026

Primer for Immunohistochemistry on Cryosectioned Rat Brain Tissue: Example Staining for Microglia and Neurons
07:30

Primer for Immunohistochemistry on Cryosectioned Rat Brain Tissue: Example Staining for Microglia and Neurons

Published on: May 12, 2015

Microglia detection by enzymatic histochemistry.

Beatriz Almolda1, Berta González, Bernardo Castellano

  • 1Universitat Autònoma de Barcelona, Barcelona, Spain.

Methods in Molecular Biology (Clifton, N.J.)
|July 2, 2013
PubMed
Summary
This summary is machine-generated.

This study presents a fast, cost-effective histochemical method for visualizing microglia in the central nervous system using nucleoside-diphosphatase (NDPase) staining. This technique aids in studying microglia

More Related Videos

Immunofluorescence Staining Using IBA1 and TMEM119 for Microglial Density, Morphology and Peripheral Myeloid Cell Infiltration Analysis in Mouse Brain
10:40

Immunofluorescence Staining Using IBA1 and TMEM119 for Microglial Density, Morphology and Peripheral Myeloid Cell Infiltration Analysis in Mouse Brain

Published on: October 27, 2019

Detection of MicroRNAs in Microglia by Real-time PCR in Normal CNS and During Neuroinflammation
13:36

Detection of MicroRNAs in Microglia by Real-time PCR in Normal CNS and During Neuroinflammation

Published on: July 23, 2012

Related Experiment Videos

Last Updated: May 10, 2026

Primer for Immunohistochemistry on Cryosectioned Rat Brain Tissue: Example Staining for Microglia and Neurons
07:30

Primer for Immunohistochemistry on Cryosectioned Rat Brain Tissue: Example Staining for Microglia and Neurons

Published on: May 12, 2015

Immunofluorescence Staining Using IBA1 and TMEM119 for Microglial Density, Morphology and Peripheral Myeloid Cell Infiltration Analysis in Mouse Brain
10:40

Immunofluorescence Staining Using IBA1 and TMEM119 for Microglial Density, Morphology and Peripheral Myeloid Cell Infiltration Analysis in Mouse Brain

Published on: October 27, 2019

Detection of MicroRNAs in Microglia by Real-time PCR in Normal CNS and During Neuroinflammation
13:36

Detection of MicroRNAs in Microglia by Real-time PCR in Normal CNS and During Neuroinflammation

Published on: July 23, 2012

Area of Science:

  • Neuroscience
  • Cell Biology
  • Histochemistry

Background:

  • Microglia visualization in the central nervous system (CNS) traditionally relies on histochemistry.
  • While immunohistochemistry is common, histochemical methods offer advantages like speed, cost-effectiveness, and cross-species applicability.
  • Specific antibodies are now preferred, but histochemistry remains a valuable tool.

Purpose of the Study:

  • To detail a histoenzymatic methodology for microglial staining using nucleoside-diphosphatase (NDPase).
  • To highlight NDPase staining's utility for visualizing diverse microglia states (amoeboid, ramified, reactive) and their relationship with blood vessels.
  • To describe adaptations for light and transmission electron microscopy, including combination with immunohistochemistry.

Main Methods:

  • Histoenzymatic demonstration of nucleoside-diphosphatase (NDPase) in microglia.
  • Application to histological sections and cell cultures for light microscopy.
  • Integration with immunohistochemistry for double labeling and adaptation for transmission electron microscopy (TEM).

Main Results:

  • NDPase staining effectively visualizes different microglia morphologies (amoeboid, ramified, reactive) in the CNS.
  • The technique also stains blood vessels, enabling analysis of microglia-vasculature interactions.
  • The method is applicable across species, including humans, and suitable for both light and electron microscopy.

Conclusions:

  • Histoenzymatic NDPase staining provides a robust, efficient method for microglial visualization in the CNS.
  • This technique complements immunohistochemistry and is valuable for studying microglia in various contexts and species.
  • The method facilitates research on microglia-CNS interactions across different developmental and pathological states.