Solid phase click ligation for the synthesis of very long oligonucleotides
Jieqiong Qiu1, Afaf H El-Sagheer, Tom Brown
1School of Chemistry, University of Southampton, Highfield, Southampton SO17 1BJ, UK.
Chemical Communications (Cambridge, England)
|July 2, 2013
Abstract:
Oligonucleotides have been ligated efficiently on solid-phase using CuAAC and SPAAC chemistry to produce up to 186-mer triazole linked DNA products. Multiple sequential ligation reactions can be carried out by using a masked azide approach. This work suggests a novel modular approach to the synthesis of large complex oligonucleotide analogues.
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