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Related Concept Videos

Preparation of Samples for Electron Microscopy01:20

Preparation of Samples for Electron Microscopy

To be visualized by an electron microscope, either transmission or scanning, biological samples need to be fixed (stabilized) so the electron beam does not destroy them and dried thoroughly (desiccated/dehydrated) so the vacuum does not affect them. Fixation needs to be done as quickly as possible because the sample properties will start changing as soon as it is removed from its natural environment. For example, in a tissue sample, the oxygen levels begin decreasing, causing an altered...

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Related Experiment Video

Updated: May 10, 2026

Dual-color Correlative Light and Electron Microscopy for the Visualization of Interactions between Mitochondria and Lysosomes
10:25

Dual-color Correlative Light and Electron Microscopy for the Visualization of Interactions between Mitochondria and Lysosomes

Published on: September 27, 2024

Single-cell lysis for visual analysis by electron microscopy.

Simon Kemmerling1, Stefan A Arnold1, Benjamin A Bircher1

  • 1Center for Cellular Imaging and Nano Analytics (C-CINA), Biozentrum, University of Basel, Basel, Switzerland.

Journal of Structural Biology
|July 3, 2013
PubMed
Summary
This summary is machine-generated.

This study introduces a novel method for lysing single cells using electrical pulses under light microscopy. The technique preserves protein structures, enabling detailed analysis with transmission electron microscopy (TEM).

Keywords:
AMBHKECLEMETElectron microscopyFEAFSHRPIDITOMSMicrofluidicsODOMPDMSPEEKRPPASNRSingle-cell analysisSingle-cell lysisSystems biologyTEMUAammonium molybdatebaby hamster kidneyddH(2)Odouble-distilled waterelectron microscopyelectron tomographyenhanced chemiluminescencefinite element analysisfused silicahorseradish peroxidaseindium tin oxideinner diametermass spectrometryoptical microscopeouter diameterpoly(dimethylsiloxane)poly(ether–ether–ketone)reverse-phase protein arrayssignal-to-noise ratiotransmission electron microscopyuranyl acetate

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Visualization of Endoplasmic Reticulum Subdomains in Cultured Cells

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Related Experiment Videos

Last Updated: May 10, 2026

Dual-color Correlative Light and Electron Microscopy for the Visualization of Interactions between Mitochondria and Lysosomes
10:25

Dual-color Correlative Light and Electron Microscopy for the Visualization of Interactions between Mitochondria and Lysosomes

Published on: September 27, 2024

Miniaturized Sample Preparation for Transmission Electron Microscopy
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Miniaturized Sample Preparation for Transmission Electron Microscopy

Published on: July 27, 2018

Visualization of Endoplasmic Reticulum Subdomains in Cultured Cells
16:43

Visualization of Endoplasmic Reticulum Subdomains in Cultured Cells

Published on: February 18, 2014

Area of Science:

  • Systems biology
  • Cell biology
  • Biophysics

Background:

  • Biological systems exhibit stochasticity, necessitating single-cell studies in systems biology.
  • Current techniques face challenges in handling single cells and analyzing low protein concentrations.
  • Transmission electron microscopy (TEM) offers single-molecule protein analysis capabilities.

Purpose of the Study:

  • To develop a novel system for single-cell lysis and sample preparation for TEM.
  • To enable the analysis of protein components within individual eukaryotic cells.
  • To overcome limitations in current single-cell handling and analysis techniques.

Main Methods:

  • Eukaryotic cells were cultured on conductively coated glass slides for light microscopy observation.
  • A custom microcapillary electrode delivered electrical pulses for targeted single-cell lysis.
  • Cell lysates were rapidly aspirated into the microcapillary and prepared for TEM analysis.

Main Results:

  • The developed method successfully achieved single-cell lysis under light microscopy.
  • The lysis and preparation process effectively conserved protein structures.
  • The method proved suitable for visual protein analysis using TEM.

Conclusions:

  • The presented system offers a viable approach for single-cell lysis and sample preparation.
  • This technique facilitates the detailed structural analysis of proteins from individual cells using TEM.
  • The method advances capabilities in systems biology by enabling single-molecule protein investigations.