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Aptamer--nanoparticle-based chemiluminescence for p53 protein
N Shwetha1, L S Selvakumar, M S Thakur
1Fermentation Technology and Bioengineering Department, CSIR-Central Food Technological Research Institute, Mysore, Karnataka 570 020, India.
Analytical Biochemistry
|July 3, 2013
Summary
This study presents a novel colorimetric biosensor for detecting the tumor suppressor protein p53 using gold nanoparticles (AuNPs). The method enhances sensitivity through a chemiluminescence reaction, improving detection limits for cancer diagnostics.
Area of Science:
- Biotechnology
- Nanotechnology
- Analytical Chemistry
Background:
- p53 is a critical tumor suppressor protein.
- Accurate detection of p53 is vital for cancer diagnosis and monitoring.
- Existing detection methods may lack sufficient sensitivity or simplicity.
Purpose of the Study:
- To develop a simple and sensitive colorimetric biosensing technique for p53 detection.
- To enhance the sensitivity of the biosensor using chemiluminescence.
- To explore the utility of gold nanoparticles (AuNPs) and aptamers in biosensing.
Main Methods:
- Utilized gold nanoparticles (AuNPs) functionalized with a p53-binding RNA aptamer.
- Induced AuNPs aggregation via aptamer-target interaction, causing a color change.
- Enhanced detection sensitivity by coupling the colorimetric method with a chemiluminescence (CL) reaction catalyzed by aggregated AuNPs.
Main Results:
- Achieved a detection limit of 0.1 ng/ml for p53 using the colorimetric approach.
- Significantly enhanced sensitivity with a CL-based method, reaching a detection limit of 10 pg/ml (R2 = 0.9907).
- Demonstrated a 10-fold increase in sensitivity compared to the colorimetric method alone.
Conclusions:
- The developed aptasensor effectively detects p53 using a colorimetric strategy.
- Combining colorimetric and CL detection significantly boosts sensitivity for p53.
- This integrated approach offers a promising platform for sensitive detection of various target analytes.
