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Electron Microscope Tomography and Single-particle Reconstruction01:07

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Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
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All-electronic Nanosecond-resolved Scanning Tunneling Microscopy: Facilitating the Investigation of Single Dopant Charge Dynamics
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Anna S. Tikhonenko: Electron microscopist extraordinary.

Vladimir I Popenko1, Elizabeth M Kutter, Hans-W Ackermann

  • 1Engelhardt Institute of Molecular Biology; Russian Academy of Sciences; Moscow, Russia.

Bacteriophage
|July 3, 2013
PubMed
Summary
This summary is machine-generated.

Anna Sergeyevna Tikhonenko pioneered bacteriophage research using electron microscopy. Her seminal book, "Ultrastructure of Bacterial Viruses," remains a fundamental resource for phage morphology and classification.

Keywords:
TEMbiographyclassificationhistoryimmuno-EM

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Area of Science:

  • Microbiology
  • Virology
  • Electron Microscopy

Background:

  • Anna Sergeyevna Tikhonenko (1925-2010) was a distinguished scientist.
  • Her work significantly advanced the field of bacteriophage research.

Purpose of the Study:

  • To highlight the contributions of Anna Sergeyevna Tikhonenko to electron microscopy and bacteriophage research.
  • To recognize the importance of her book, "Ultrastructure of Bacterial Viruses".

Main Methods:

  • Electron microscopy was the primary technique used.
  • Detailed morphological analysis of bacteriophages was conducted.

Main Results:

  • Tikhonenko published 113 articles and one book on bacterial viruses.
  • Her book featured 134 high-quality micrographs of 316 phages.
  • She proposed a morphological classification of phages into five family-level categories.

Conclusions:

  • Tikhonenko's electron microscopical work, especially on bacteriophages, was excellent.
  • Her book, "Ultrastructure of Bacterial Viruses," was the first bacteriophage atlas and remains a fundamental resource.
  • Her classification system contributed significantly to phage research.