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Published on: August 26, 2021
Cytotoxic activity and apoptosis induction by gaillardin
Maryam Hamzeloo Moghadam1, Farzaneh Naghibi, Azadeh Atoofi
1Department of Traditional Pharmacy, School of Traditional Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Summary
Gaillardin, a compound from Inula oculus-christi, shows significant cytotoxic activity against various cancer cell lines, including breast and colon cancer. Further research is recommended for its potential in cancer therapy.
Area of Science:
- Natural Product Chemistry
- Cancer Biology
- Pharmacology
Background:
- Sesquiterpene lactones are a class of natural compounds with diverse biological activities.
- Inula oculus-christi L. (Asteraceae) is a plant species known to contain various bioactive compounds.
- Cancer remains a leading cause of mortality worldwide, necessitating the search for novel therapeutic agents.
Purpose of the Study:
- To evaluate the cytotoxic activity of gaillardin, a sesquiterpene lactone from Inula oculus-christi, against human cancer cell lines.
- To investigate the in vitro apoptosis-inducing properties of gaillardin.
Main Methods:
- Cytotoxicity was assessed using the microculture tetrazolium-formazan MTT assay.
- Apoptosis induction was evaluated in MCF-7 cells using the terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling (TUNEL) assay.
Main Results:
- Gaillardin exhibited cytotoxic activity against MCF-7, HepG-2, A-549, and HT-29 cancer cell lines with IC50 values ranging from 1.81 to 6.37 microg/mL.
- The compound demonstrated in vitro apoptosis-inducing properties in MCF-7 cells.
Conclusions:
- Gaillardin possesses significant cytotoxic and apoptosis-inducing effects on cancer cells.
- Gaillardin is a promising candidate for further investigation in cancer therapy development.
