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Electrospun Nanofiber Scaffolds with Gradations in Fiber Organization
Published on: April 19, 2015
Biocompatibility evaluation of emulsion electrospun nanofibers using osteoblasts for bone tissue engineering
Lingling Tian1, Molamma P Prabhakaran, Xin Ding
1a Key Laboratory of Textile Science & Technology, Ministry of Education of China , Donghua University , Shanghai , 201620 , China .
Journal of Biomaterials Science. Polymer Edition
|July 4, 2013
Summary
Dual-factor nanofibers combining hydroxyapatite (HA) and laminin show enhanced osteoblast response. This emulsion electrospinning technique creates advanced scaffolds for bone tissue regeneration.
Area of Science:
- Biomaterials Science
- Tissue Engineering
- Nanotechnology
Background:
- Core-shell nanofibrous scaffolds fabricated via emulsion electrospinning offer potential for drug encapsulation and tissue regeneration.
- Incorporating multiple bioactive factors can synergistically enhance cellular responses compared to single-factor systems.
Purpose of the Study:
- To fabricate and evaluate poly(L-lactic acid-co-ε-caprolactone) (PLCL) based core-shell nanofibers co-delivering hydroxyapatite (HA) and laminin (Lam) for bone tissue engineering.
- To investigate the synergistic effects of HA and Lam on osteoblast adhesion, proliferation, maturation, and mineralization.
Main Methods:
- Fabrication of PLCL/HA, PLCL/Lam, and PLCL/HA/Lam scaffolds using emulsion electrospinning.
- Characterization of scaffold morphology, fiber diameter, and elastic modulus.
- In vitro evaluation of osteoblast behavior including proliferation, alkaline phosphatase (ALP) activity, and calcium deposition.
Main Results:
- PLCL/HA/Lam scaffolds exhibited fiber diameters of 379 ± 57 nm and elastic moduli ranging from 22.7-37.0 MPa.
- Osteoblast proliferation was significantly higher on PLCL/HA/Lam scaffolds compared to single-factor scaffolds.
- Enhanced cell maturation (ALP activity) and calcium deposition were observed on PLCL/HA/Lam scaffolds, indicating improved osteogenic differentiation.
Conclusions:
- Laminin and HA individually promote osteoblast proliferation and maturation.
- The synergistic combination of HA and Lam in PLCL/HA/Lam nanofibers significantly enhances osteoblast functionality.
- These novel scaffolds hold promise for applications in bone tissue regeneration.
