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DNA Isolation

DNA Isolation

DNA from cells is required for many biotechnology and research applications, such as molecular cloning. To remove and purify DNA from cells, researchers use various methods of DNA extraction. While the specifics of different protocols may vary, some general concepts underlie the process of DNA extraction.

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Related Experiment Video

Updated: May 10, 2026

A Simple, Quick, and Partially Automated Protocol for the Isolation of Single Nuclei from Frozen Mammalian Tissues for Single Nucleus Sequencing

A Simple, Quick, and Partially Automated Protocol for the Isolation of Single Nuclei from Frozen Mammalian Tissues for Single Nucleus Sequencing

Published on: July 28, 2023

RNA isolation from mammalian samples.

Ximeng Liu¹, Shuko Harada

¹Department of Pathology, University of Alabama at Birmingham, Birmingham, AL, USA.

Current Protocols in Molecular Biology

|July 4, 2013

Summary

This summary is machine-generated.

This study details methods for extracting RNA from various biological samples, including tissues and bodily fluids. It presents two primary RNA isolation protocols: one using phenol/chloroform extraction and another employing silica-column separation.

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Last Updated: May 10, 2026

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Isolation of Small Noncoding RNAs from Human Serum

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Published on: June 19, 2014

Area of Science:

Molecular Biology
Biochemistry

Background:

RNA extraction is crucial for molecular analyses.
Diverse biological sources yield RNA, including cells, tissues, and body fluids.
Formalin-fixed paraffin-embedded (FFPE) tissues are a valuable RNA source.

Purpose of the Study:

To describe established methods for RNA isolation.
To present two distinct RNA extraction protocols.

Main Methods:

Phenol/chloroform extraction.
Silica spin-column absorption.
Isopycnic gradient centrifugation.
A one-step monophasic lysis protocol using guanidine isothiocyanate, phenol, and chloroform.
A silica-column separation method for RNA isolation.

Main Results:

RNA can be successfully extracted from a wide array of biological specimens.
Two fundamental RNA isolation procedures are detailed, offering different approaches.
The described methods facilitate the purification of RNA for downstream applications.

Conclusions:

Effective RNA extraction is achievable from numerous sample types.
The choice of RNA isolation method depends on the sample source and downstream requirements.
Detailed protocols are provided for phenol/chloroform and silica-column RNA isolation.