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Published on: February 23, 2014
The engraftment and differentiation of transplanted bone marrow-derived cells in the olfactory bulb after methimazole
Yohei Noda1, Kazunori Nishizaki, Junko Yoshinobu
1Department of Otolaryngology-Head & Neck Surgery, Okayama, Japan.
Conclusion:
Bone marrow-derived cells can be engrafted in the olfactory bulb and a few cells can differentiate into mitral/tufted cells in the olfactory bulb.
Objectives:
To investigate whether bone marrow-derived cells can be engrafted into the olfactory bulb and differentiate into neurons and glial cells after methimazole administration.
Methods:
Bone marrow of GFP (green fluorescence protein) mice was transplanted into lethally irradiated recipient mice. Immunostaining was performed to confirm the cell types of bone marrow-derived cells expressing GFP.
Results:
GFP-positive cells were observed in the olfactory bulb at 2 days after methimazole administration. The number of dendritic GFP-positive cells increased up to 30 days after methimazole administration and then decreased. Double immunostaining for GFP and Iba1 or TBX21 showed that a large population of the GFP-positive cells had characteristics of microglia/macrophages and a few cells had characteristics of mitral/tufted cells.
