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Updated: May 10, 2026

Amplicon Sequencing using the Long-Read Sequencing Technologies

Amplicon Sequencing using the Long-Read Sequencing Technologies

Published on: August 29, 2025

The advantages of SMRT sequencing.

Richard J Roberts, Mauricio O Carneiro, Michael C Schatz

|July 5, 2013

Summary

Single-molecule, real-time (SMRT) sequencing offers long reads, modified base detection, and high accuracy. This makes SMRT sequencing an ideal approach for the complete sequencing of small genomes.

Area of Science:

Genomics
Molecular Biology
Next-Generation Sequencing

Background:

Next-generation sequencing (NGS) technologies are rapidly advancing.
Single-molecule, real-time (SMRT) sequencing is a notable NGS technology.
SMRT sequencing is often overlooked despite its capabilities.

Discussion:

SMRT sequencing provides long reads, enabling comprehensive genome assembly.
It offers direct detection of modified bases, crucial for epigenomic studies.
High accuracy of SMRT sequencing ensures reliable variant calling.

Key Insights:

SMRT sequencing is a powerful tool for small genome sequencing.
Its long reads facilitate the complete assembly of complex genomes.
Modified base detection adds another layer of biological insight.

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Last Updated: May 10, 2026

Amplicon Sequencing using the Long-Read Sequencing Technologies

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Published on: August 29, 2025

Selective Capture of 5-hydroxymethylcytosine from Genomic DNA

Selective Capture of 5-hydroxymethylcytosine from Genomic DNA

Published on: October 5, 2012

Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations

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Published on: August 4, 2016

Outlook:

Further adoption of SMRT sequencing for small genome projects is anticipated.
Integration of SMRT data with other NGS platforms may enhance genomic analyses.
Continued technological advancements will likely expand SMRT sequencing applications.