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Related Concept Videos

Protein Networks02:26

Protein Networks

An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
Protein Networks02:26

Protein Networks

An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
Protein-protein Interfaces02:04

Protein-protein Interfaces

Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a polypeptide...
Protein-Protein Interfaces02:04

Protein-Protein Interfaces

Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a polypeptide...
Introduction to Innate and Adaptive Immunity01:21

Introduction to Innate and Adaptive Immunity

The human immune system is a complex defense mechanism that protects the body from harmful pathogens and foreign substances. It comprises two crucial components: innate and adaptive immunity.
Innate immunity is the body's natural, nonspecific defense system that acts quickly to protect against pathogens. It incorporates physical barriers like skin and mucous membranes and cellular elements such as phagocytes and natural killer cells. This part of our immune system provides an immediate,...
NF-κB-dependent Signaling Pathway02:26

NF-κB-dependent Signaling Pathway

The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The heterodimer of NF-κB...

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Related Experiment Video

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Quantification of Protein Interaction Network Dynamics using Multiplexed Co-Immunoprecipitation
07:57

Quantification of Protein Interaction Network Dynamics using Multiplexed Co-Immunoprecipitation

Published on: August 21, 2019

Protein interaction networks in innate immunity.

Manuele Rebsamen1, Richard Kumaran Kandasamy, Giulio Superti-Furga

  • 1CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences, Vienna, Austria.

Trends in Immunology
|July 6, 2013
PubMed
Summary

Understanding the immune system requires studying molecular networks. Proteomics technologies help identify protein complexes crucial for pathogen recognition and host defense, revealing how cells maintain homeostasis.

Keywords:
innate immunitymass spectrometryprotein complexproteomicssystems biology

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Visualization of Protein-protein Interaction in Nuclear and Cytoplasmic Fractions by Co-immunoprecipitation and In Situ Proximity Ligation Assay
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Area of Science:

  • Immunology and Molecular Biology
  • Proteomics and Systems Biology

Background:

  • Immune responses to pathogens are governed by intricate molecular networks.
  • Technological advancements enable systems-level analysis of innate immunity and host-pathogen interactions.
  • Protein complexes are central to pathogen recognition and integrating host responses.

Purpose of the Study:

  • To review current proteomics technologies for identifying protein complexes in immune signaling.
  • To highlight studies demonstrating the application of proteomics in understanding host-pathogen interactions.
  • To illustrate how protein complex analysis contributes to understanding cellular homeostasis.

Main Methods:

  • Focus on proteomics technologies for protein complex identification.
  • Review of recent studies utilizing these proteomic approaches.
  • Analysis of protein complex dynamics, abundance, and composition.

Main Results:

  • Proteomics offers powerful tools to identify protein complexes involved in innate immunity.
  • These technologies enable monitoring of host-pathogen interactions at a systems level.
  • Studies reveal the integration of immune machinery with cellular processes.

Conclusions:

  • Monitoring protein complex characteristics is vital for understanding cellular immune responses.
  • Proteomics is instrumental in unraveling the molecular basis of host defense and homeostasis.
  • Systems-level insights from proteomics advance our comprehension of immune regulation.