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HPLC-DAD method for comprehensive quality control of Semen Strychni
Huai-Bo Tang1, Hua-Lin Cai, Huan-De Li
1Clinical Pharmacy and Pharmacology Research Institute, Pharmaceutical Department, the Second Xiangya Hospital of Central South University , Changsha , China and.
Pharmaceutical Biology
|July 9, 2013
Summary
A new method uses high-performance liquid chromatography to control the quality of Semen Strychni. It identifies key compounds like chlorogenic acid and loganin for accurate authentication and quality assessment.
Area of Science:
- Pharmacognosy
- Analytical Chemistry
- Traditional Chinese Medicine
Background:
- Semen Strychni, derived from Strychnos nux-vomica L., faces quality control challenges due to a narrow focus on alkaloids.
- Chlorogenic acid and loganin are identified as novel marker constituents, selected for their bioactive properties linked to therapeutic effects.
Purpose of the Study:
- To establish a straightforward and comprehensive method for the quantitative quality control of Semen Strychni.
- To validate the use of chlorogenic acid and loganin as primary quality control markers.
Main Methods:
- Optimal ultrasonic extraction was performed using 50% aqueous methanol with 1% formic acid for 45 minutes.
- High-performance liquid chromatography (HPLC) with a diode array detector was employed for separation and quantification.
- The HPLC method was rigorously validated for linearity, LOQ, precision, repeatability, accuracy, and stability.
Main Results:
- Excellent linearity was observed for all calibration curves (r² ≥ 0.999).
- The method demonstrated high reproducibility and accuracy, with RSD values below 4.55% and accuracy ranging from 95.6% to 104.4%.
- The validated method was successfully applied to quantify components in different parts of Semen Strychni, including seed coat, leaf, endosperm, and whole seed.
Conclusions:
- A validated, simple, and practical HPLC method has been developed for Semen Strychni quality control.
- This method provides a reliable tool for the authentication and quality assessment of Semen Strychni, incorporating novel marker compounds.
