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Updated: May 10, 2026
Identification of Functional Protein Regions Through Chimeric Protein Construction
Published on: January 8, 2019
Construction and characterization of a new chimeric antibody against HER2
Mohammad Mehdi Amiri1, Mahmood Jeddi-Tehrani, Tohid Kazemi
1Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
Aim:
Immunotherapy with anti-HER2 antibodies has shown promising results in patients with HER2-positive breast cancer. We have recently reported characterization of a mouse monoclonal antibody (mAb) against HER2, which binds to an epitope different from that recognized by trastuzumab and specifically inhibits proliferation of tumor cells overexpressing HER2. In the present study we report chimerization of this antibody.
Materials & Methods:
The immunoglobulin variable region heavy and light chain genes of 1T0 hybridoma cells were amplified and ligated to human γ-1 and κ constant region genes using splice overlap extension PCR. The chimeric antibody was subsequently expressed and characterized by ELISA, western blot and flow cytometry.
Results:
The purified chimeric antibody specifically binds to recombinant HER2 and HER2-overexpressing tumor cells and inhibits proliferation of these cells. The binding affinity of the chimeric mAb was comparable with the parental mouse mAb.
Conclusion:
This chimeric anti-HER2 mAb is a potentially valuable tool for targeted immunotherapy.
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