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Updated: May 10, 2026

Laser Micro-Irradiation to Study DNA Recruitment During S Phase
Published on: April 16, 2021
The differences between ICL repair during and outside of S phase
Hannah L Williams1, Max E Gottesman, Jean Gautier
1Department of Genetics and Development, Institute for Cancer Genetics, Columbia University, New York, NY 10032, USA.
Abstract:
DNA interstrand crosslinks (ICLs) are complex lesions that block essential DNA transactions including DNA replication, recombination, and RNA transcription. Naturally occurring ICLs are rare, yet these lesions are the major cause of toxicity following treatment with several classes of crosslinking cancer chemotherapeutic drugs. ICLs are repaired during and outside of S phase by pathways with overlapping as well as distinct features. Here, we discuss some recent insights into the mechanisms of replication-dependent and replication-independent repair of ICLs with special emphasis on the differences between these repair pathways.
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