Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: May 9, 2026

Viability Assays for Cells in Culture
12:03

Viability Assays for Cells in Culture

Published on: January 20, 2014

Kinetic viability assays using DRAQ7 probe.

Donald Wlodkowic1,2, Jin Akagi1, Jurek Dobrucki3

  • 1The BioMEMS Research Group, School of Chemical Sciences, University of Auckland, Auckland, New Zealand.

Current Protocols in Cytometry
|July 10, 2013
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Modulation of CREB signaling contributes to anxiety-like phenotypes in a kindling model of adult zebrafish.

Experimental neurology·2026
Same author

(<i>E</i>)-4-(4-Acrylamidophenoxy)-<i>N</i>-Methylpicolinamides as b-Raf/VEGFR-2 Inhibitors with Antiangiogenic Activity in HUVEC and Zebrafish Model.

Molecules (Basel, Switzerland)·2026
Same author

Thermotactic Decision-Making in Aquatic Invertebrates: High-Resolution Behavioral Analysis of Ecotoxicological Effects.

Environmental science & technology·2026
Same author

The neural impacts of gun violence exposure on adult attentional mechanisms: an EEG pilot study.

Cogent mental health·2026
Same author

Organic pollutants leaching from tire waste: Ecotoxicity implications for aquatic species.

Aquatic toxicology (Amsterdam, Netherlands)·2025
Same author

Real-time assessment of circulating tumor cells refines the indication for HER2-targeted therapy in metastatic gastric cancer.

Scientific reports·2025
Same journal

Detection and Sorting of Extracellular Vesicles and Viruses Using nanoFACS.

Current protocols in cytometry·2020
Same journal

Live Imaging of the Lung.

Current protocols in cytometry·2020
Same journal

Small Particle Fluorescence and Light Scatter Calibration Using FCM<sub>PASS</sub> Software.

Current protocols in cytometry·2020
Same journal

Optimized Stochastic Optical Reconstruction Microscopy for Imaging Chromatin Structure in Pathological Tissue.

Current protocols in cytometry·2020
Same journal

Flow Cytometric Quantification of Granulocytic Alkaline Phosphatase Activity in Unlysed Whole Blood.

Current protocols in cytometry·2020
Same journal

Practical Guidelines for Collection, Manipulation and Inactivation of SARS-CoV-2 and COVID-19 Clinical Specimens.

Current protocols in cytometry·2020
See all related articles
This summary is machine-generated.

This study introduces DRAQ7, a novel fluorescent probe for real-time cell death monitoring. It enables dynamic tracking of cell viability, overcoming limitations of traditional end-point assays for stochastic cell death processes.

Area of Science:

  • Cell biology
  • Biotechnology
  • Molecular diagnostics

Background:

  • Cell death is a stochastic process with asynchronous progression through death stages.
  • Current cell death assays lack the ability to track dynamic, real-time cell responses.
  • There is a need for high-throughput bioassays to continuously monitor individual cell viability.

Purpose of the Study:

  • To introduce and validate the use of DRAQ7 for real-time cell death monitoring.
  • To provide protocols for different DRAQ7-based viability assays.
  • To enable dynamic tracking of cell death and apoptosis.

Main Methods:

  • Development of three DRAQ7-based cell viability assay protocols.
  • Real-time single-color DRAQ7 assay for dynamic viability tracking.

More Related Videos

Kinetic Screening of Nuclease Activity using Nucleic Acid Probes
06:52

Kinetic Screening of Nuclease Activity using Nucleic Acid Probes

Published on: November 1, 2019

Quantitative, Real-time Analysis of Base Excision Repair Activity in Cell Lysates Utilizing Lesion-specific Molecular Beacons
15:01

Quantitative, Real-time Analysis of Base Excision Repair Activity in Cell Lysates Utilizing Lesion-specific Molecular Beacons

Published on: August 6, 2012

Related Experiment Videos

Last Updated: May 9, 2026

Viability Assays for Cells in Culture
12:03

Viability Assays for Cells in Culture

Published on: January 20, 2014

Kinetic Screening of Nuclease Activity using Nucleic Acid Probes
06:52

Kinetic Screening of Nuclease Activity using Nucleic Acid Probes

Published on: November 1, 2019

Quantitative, Real-time Analysis of Base Excision Repair Activity in Cell Lysates Utilizing Lesion-specific Molecular Beacons
15:01

Quantitative, Real-time Analysis of Base Excision Repair Activity in Cell Lysates Utilizing Lesion-specific Molecular Beacons

Published on: August 6, 2012

  • Endpoint DRAQ7 staining and real-time multiparametric apoptosis assay with TMRM.

    • Main Results:

    • DRAQ7 is a non-penetrating probe that labels DNA in cells with compromised membrane integrity.
    • Protocols enable real-time monitoring of cell death dynamics.
    • Multiparametric assays combine DRAQ7 with mitochondrial potential probes for apoptosis studies.

    Conclusions:

    • DRAQ7 is a versatile tool for real-time and endpoint cell death assays.
    • The developed protocols facilitate dynamic monitoring of cell viability and apoptosis.
    • DRAQ7 offers an improved method for studying stochastic cell death processes.