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Updated: May 9, 2026

Tea Aroma Analysis Based on Solvent-Assisted Flavor Evaporation Enrichment
Published on: May 26, 2023
A comparative study of volatile components in green, oolong and black teas by using comprehensive two-dimensional gas
Lei Zhang1, Zhongda Zeng, Chunxia Zhao
1CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
Abstract:
The difference of volatile components in green, oolong and black teas was studied by using comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry (GC×GC-TOFMS). Simultaneous distillation extraction was proved to be a suitable technique to extract the analytes with interest. A total of 450 compounds were tentatively identified with comparison to the standard mass spectra in available databases, retention index on the first dimension and structured chromatogram. 33 tea samples, including 12, 12 and 9 samples of green, oolong and black tea were analyzed by using GC×GC-TOFMS. After peak alignment, around 3600 peaks were detected. Partial least squares - discriminant analysis and hierarchical cluster analysis were used to classify these samples, then non-parametric hypothesis test (Mann-Whitney U test) and the variable importance in the projection (VIP) were applied to discover the key components to distinguish the three types of tea with significant difference amongst them. 74 differential compounds are defined to interpret the chemical differences of 3 types of tea. This study shows the power of GC×GC-TOFMS method combined with multivariate data analysis to investigate natural products with high complexity for information extraction.
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