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Related Concept Videos

DNA Replication02:40

DNA Replication

DNA replication involves the separation of the two strands of the double helix, with each strand serving as a template from which the new complementary strand is copied.  After replication, each double-stranded DNA includes one parental or “old” strand and one “new” strand. This is known as semiconservative replication. The resulting DNA molecules have the same sequence and are divided equally into the two daughter cells.
Replication in Prokaryotes
DNA replication uses a large number of...
Replication in Eukaryotes01:29

Replication in Eukaryotes

In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...
Replication in Eukaryotes02:31

Replication in Eukaryotes

Overview
Replication in Eukaryotes02:31

Replication in Eukaryotes

Overview
Replication in Eukaryotes01:29

Replication in Eukaryotes

In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...
Chromosome Replication02:31

Chromosome Replication

Before a cell can divide, it must accurately replicate all of its chromosomes, including the DNA and its associated histone and non-histone proteins.  This process begins at numerous origins of replication during the S phase of the cell cycle in each of a cell’s chromosomes simultaneously. Certain nucleotides can act as origins of replication, but these sequences are not well defined - especially in complex, multi-cellular, eukaryotic species. The length of DNA that spans an origin of...

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Updated: May 9, 2026

Genome-wide Determination of Mammalian Replication Timing by DNA Content Measurement
08:06

Genome-wide Determination of Mammalian Replication Timing by DNA Content Measurement

Published on: January 19, 2017

DNA replication timing.

Nicholas Rhind1, David M Gilbert

  • 1Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA. nick.rhind@umassmed.edu

Cold Spring Harbor Perspectives in Biology
|July 11, 2013
PubMed
Summary
This summary is machine-generated.

Genome replication timing correlates with chromosome structure, suggesting domain-level control. This research explores how chromosomal domains influence DNA replication patterns in eukaryotes.

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Last Updated: May 9, 2026

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Area of Science:

  • Genomics
  • Molecular Biology
  • Epigenetics

Background:

  • Replication patterns in eukaryotic genomes are linked to gene expression, chromatin structure, and evolution.
  • Advances in genome-scale replication kinetics mapping enable exploration across species, cell types, and conditions.
  • Quantitative and computational analyses of large datasets reveal new genomic correlations.

Purpose of the Study:

  • To investigate the correlation between replication timing and the three-dimensional structure of chromosomes.
  • To explore the role of chromosomal domains in controlling replication timing.
  • To propose a model for replication pattern generation based on domain structure and origin firing.

Main Methods:

  • Genome-scale mapping of replication kinetics.
  • Quantitative and computational analyses of large genomic datasets.
  • Comparative analysis across species, cell types, and growth conditions.

Main Results:

  • A strong correlation was identified between replication timing and chromosome three-dimensional structure.
  • This correlation is stronger than with individual histone modifications or chromosome-binding proteins.
  • Replication timing appears to be controlled at the level of chromosomal domains.

Conclusions:

  • Chromosomal domain structure plays a significant role in modulating the probability of origin firing.
  • A model is proposed where domain structure influences stochastic origin firing to generate replication patterns.
  • The functional significance of these replication patterns, whether inherent or reflective of genome organization, remains an open question.