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Semi-quantitative analysis of transcript accumulation in response to drought stress by Lepidium latifolium seedlings
Sanjay Mohan Gupta1, Sadhana Singh, Pankaj Pandey
1Molecular Biology and Genetic Engineering Laboratory; Defence Institute of Bio-Energy Research; DRDO; Haldwani, India; These authors contributed equally to this work.
Abstract:
Cross-amplification of five Arabidopsis abiotic stress-responsive genes (AtPAP, ZFAN, Vn, LC4 and SNS) in Lepidium has been documented in plants raised out of seeds pre-treated with potassium nitrate (KNO 3) for assessment of enhanced drought stress tolerance. cDNA was synthesized from Lepidium plants pre-treated with KNO 3 (0.1% and 0.3%) and exposed to drought conditions (5% and 15% PEG) at seedling stage for 30 d. Transcript accumulation of all the five genes were found suppressed in set of seedlings, which were pre-treated with 0.1% KNO 3 and were exposed to 15% PEG for 30 d. The present study establishes that different pre-treatments may further enhance the survivability of Lepidium plants under conditions of drought stress to different degrees.
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