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A fluorogenic assay for screening Sirt6 modulators.

Jing Hu1, Bin He, Shiva Bhargava

  • 1Department of Chemistry and Chemical Biology, Baker Laboratory, Cornell University, Ithaca, NY 14853, USA.

Organic & Biomolecular Chemistry
|July 11, 2013
PubMed
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A new high-throughput assay screens for Sirt6 modulators by measuring its ability to break down myristoyl lysine. This tool aids research into Sirt6 function and protein lysine fatty acylation.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Enzymology

Background:

  • Sirtuin 6 (Sirt6) is an enzyme involved in various cellular processes.
  • Protein lysine fatty acylation is a regulatory modification with emerging biological significance.
  • Understanding Sirt6's enzymatic activity is crucial for deciphering its roles.

Purpose of the Study:

  • To develop a fluorogenic high-throughput assay for screening Sirt6 modulators.
  • To leverage the known activity of Sirt6 in hydrolyzing myristoyl lysine.
  • To provide a tool for investigating Sirt6 function and protein lysine fatty acylation.

Main Methods:

  • Development of a fluorogenic assay.
  • Utilizing myristoyl lysine as a substrate for Sirt6.
  • High-throughput screening methodology.

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Published on: February 27, 2016

Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy
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Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy

Published on: January 18, 2017

Related Experiment Videos

Last Updated: May 9, 2026

A Fluorescent Screening Assay for Identifying Modulators of GIRK Channels
05:31

A Fluorescent Screening Assay for Identifying Modulators of GIRK Channels

Published on: April 24, 2012

Deacetylation Assays to Unravel the Interplay between Sirtuins (SIRT2) and Specific Protein-substrates
14:32

Deacetylation Assays to Unravel the Interplay between Sirtuins (SIRT2) and Specific Protein-substrates

Published on: February 27, 2016

Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy
09:30

Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy

Published on: January 18, 2017

Main Results:

  • A robust assay capable of detecting Sirt6 activity was established.
  • The assay is suitable for screening potential Sirt6 modulators.
  • Demonstrated Sirt6's efficient hydrolysis of myristoyl lysine.

Conclusions:

  • The developed assay is a valuable tool for Sirt6 research.
  • Sirt6 modulators identified using this assay will advance the study of Sirt6.
  • This work facilitates deeper understanding of protein lysine fatty acylation.