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Regulated expression of PTPRJ/CD148 and an antisense long noncoding RNA in macrophages by proinflammatory stimuli
Richa K Dave1, Marcel E Dinger, Megan Andrew
1The University of Queensland, Institute for Molecular Bioscience, Brisbane, Australia.
Abstract:
PTPRJ/CD148 is a tyrosine phosphatase that has tumour suppressor-like activity. Quantitative PCR of various cells and tissues revealed that it is preferentially expressed in macrophage-enriched tissues. Within lymphoid tissues immunohistochemistry revealed that PTPRJ/CD148 co-localised with F4/80, indicating that macrophages most strongly express the protein. Macrophages express the highest basal level of ptprj, and this is elevated further by treatment with LPS and other Toll-like receptor ligands. In contrast, CSF-1 treatment reduced basal and stimulated Ptprj expression in human and mouse cells, and interferon also repressed Ptprj expression. We identified a 1006 nucleotide long noncoding RNA species, Ptprj-as1 that is transcribed antisense to Ptprj. Ptprj-as1 was highly expressed in macrophage-enriched tissue and was transiently induced by Toll-like receptor ligands with a similar time course to Ptprj. Finally, putative transcription factor binding sites in the promoter region of Ptprj were identified.
Insights
The tyrosine phosphatase PTPRJ/CD148 is highly expressed in macrophages and its expression is regulated by immune stimuli. A novel antisense noncoding RNA, Ptprj-as1, was identified and also found to be regulated by immune signaling.
Area of Science:
- Immunology
- Molecular Biology
- Cancer Research
Background:
- PTPRJ/CD148 is a tyrosine phosphatase with tumor suppressor-like activity.
- Its expression patterns and regulatory mechanisms are not fully understood, particularly in immune cells.
Purpose of the Study:
- To investigate the expression profile and regulation of PTPRJ/CD148 in macrophages.
- To identify potential regulatory elements, including noncoding RNAs, involved in PTPRJ/CD148 expression.
Main Methods:
- Quantitative PCR (qPCR) to assess gene expression in various tissues and cell types.
- Immunohistochemistry to determine protein localization within tissues.
- Treatment of cells with various stimuli (LPS, TLR ligands, CSF-1, interferon) to study gene regulation.
- Identification and characterization of a novel antisense noncoding RNA (Ptprj-as1).
Main Results:
- PTPRJ/CD148 is preferentially expressed in macrophage-enriched tissues and co-localizes with F4/80.
- Macrophage PTPRJ/CD148 expression is upregulated by LPS and Toll-like receptor ligands but repressed by CSF-1 and interferon.
- A novel antisense noncoding RNA, Ptprj-as1, was identified, highly expressed in macrophages, and similarly induced by TLR ligands.
- Putative transcription factor binding sites in the PTPRJ promoter region were identified.
Conclusions:
- Macrophages are a primary site of PTPRJ/CD148 expression, with its levels dynamically regulated by immune stimuli.
- The novel antisense noncoding RNA Ptprj-as1 may play a role in regulating PTPRJ expression in response to immune activation.
- Understanding PTPRJ/CD148 regulation provides insights into macrophage function and potential therapeutic strategies.
