Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Matrix-Assisted Laser Desorption Ionization (MALDI)

Matrix-Assisted Laser Desorption Ionization (MALDI)

Matrix-assisted laser desorption ionization (MALDI) is a powerful analytical technique used in mass spectrometry. It enables the identification and characterization of various biomolecules, including proteins, peptides, nucleic acids, and carbohydrates. MALDI is an ionization technique, widely employed in biological and medical research, as well as in fields like pharmacology and biochemistry.The analyte of interest, a biomolecule or a mixture of biomolecules, is mixed with a suitable matrix...

MALDI-TOF Mass Spectrometry

MALDI-TOF Mass Spectrometry

Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...

Peptide Identification Using Tandem Mass Spectrometry

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...

Tandem Mass Spectrometry

Tandem Mass Spectrometry

Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...

Mass Spectrometry: Complex Analysis

Mass Spectrometry: Complex Analysis

Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Plasmonic Nanocavity-Induced Degradation Pathway of Boronic Acid Biosensing Interfaces Revealed by <i>In Situ</i> Tip-Enhanced Raman Spectroscopy.

ACS nano·2026

Same author

Noninvasive Analysis of Skin Emanations during Cupping Therapy by Thin-Film Solid-Phase Microextraction and Dielectric Barrier Discharge Ionization Mass Spectrometry.

Analytical chemistry·2026

Same author

Tracking Protein Misfolding and Oligomerization: A Temperature-Controlled Ion Mobility-Mass Spectrometry Approach.

Analytical chemistry·2026

Same author

Integrating Functional Response and Target Binding for Mechanism-Centered Drug Screening by High-Mass MALDI-MS.

ACS central science·2026

Same author

Engineered Strain in 2D Materials by Direct Growth on Deterministically Patterned Grayscale Topographies.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026

Same author

Nanoscale Chemical Analysis of Heterogeneous Catalysts Using Tip-Enhanced Raman Spectroscopy.

Chemical reviews·2026

Search research articles

Related Experiment Video

Updated: May 9, 2026

Dithranol as a Matrix for Matrix Assisted Laser Desorption/Ionization Imaging on a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer

Dithranol as a Matrix for Matrix Assisted Laser Desorption/Ionization Imaging on a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer

Published on: November 26, 2013

Matrix-assisted laser desorption/ionization matrices for negative mode metabolomics.

Stephan R Fagerer¹, Simone Nielsen, Alfredo Ibáñez

¹Department of Chemistry and Applied Biosciences, ETH Zurich, CH-8093 Zurich, Switzerland.

European Journal of Mass Spectrometry (Chichester, England)

|July 12, 2013

Summary

Researchers explored new matrices for matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) in metabolomics. Ethacridine lactate and 4-amino-2-methylquinoline show promise as alternatives to 9-aminoacridine (9AA), offering improved detection limits and broader analyte coverage.

More Related Videos

Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry (UPLC-MS)

Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry (UPLC-MS)

Published on: March 14, 2013

MALDI-Mass Spectrometric Imaging for the Investigation of Metabolites in Medicago truncatula Root Nodules

MALDI-Mass Spectrometric Imaging for the Investigation of Metabolites in Medicago truncatula Root Nodules

Published on: March 5, 2014

Related Experiment Videos

Last Updated: May 9, 2026

Dithranol as a Matrix for Matrix Assisted Laser Desorption/Ionization Imaging on a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer

Dithranol as a Matrix for Matrix Assisted Laser Desorption/Ionization Imaging on a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer

Published on: November 26, 2013

Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry (UPLC-MS)

Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry (UPLC-MS)

Published on: March 14, 2013

MALDI-Mass Spectrometric Imaging for the Investigation of Metabolites in Medicago truncatula Root Nodules

MALDI-Mass Spectrometric Imaging for the Investigation of Metabolites in Medicago truncatula Root Nodules

Published on: March 5, 2014

Area of Science:

Analytical Chemistry
Biochemistry
Mass Spectrometry

Background:

Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) is crucial for metabolomics.
9-aminoacridine (9AA) is a common matrix but has limitations like interferences and variable sensitivity.
Discovering alternative matrices is essential for broader metabolite identification.

Purpose of the Study:

To evaluate novel matrices for negative mode MALDI-MS in metabolomics.
To compare the performance of ethacridine lactate and 4-amino-2-methylquinoline against 9AA.
To assess the limits of detection (LODs) and analyte coverage of tested matrices.

Main Methods:

Tested a series of potential negative mode MALDI matrices.
Utilized a mixture of 29 diverse metabolites (amino acids, nucleotide phosphates, Krebs cycle intermediates).
Determined limits of detection (LODs) for each metabolite with different matrices.

Main Results:

Ethacridine lactate achieved low femtomole LODs for nucleotide phosphates and low picomole LODs for amino acids and Krebs cycle intermediates.
4-amino-2-methylquinoline demonstrated picomole LODs for most metabolites and broader analyte ionization than 9AA and ethacridine.
Both tested matrices showed potential advantages over the standard 9AA matrix.

Conclusions:

Ethacridine lactate and 4-amino-2-methylquinoline are promising alternative matrices for negative mode MALDI-MS in metabolomics.
These matrices offer improved sensitivity and/or broader analyte coverage compared to 9AA.
Further research with these matrices could enhance metabolite profiling and discovery.