A census of RND superfamily proteins in the Burkholderia genus
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Updated: May 9, 2026

Total Protein Extraction and 2-D Gel Electrophoresis Methods for Burkholderia Species
Published on: October 15, 2013
Elena Perrin1, Marco Fondi, Maria Cristiana Papaleo
1Laboratory of Molecular & Microbial Evolution, Department of Biology, University of Florence, Via Madonna del Piano 6, 50019 Sesto Fiorentino (Fi), Italy.
Aim:
The aim of this work was to analyze the eight resistance-nodulation-cell division (RND) families (a group of proteins mainly involved in multidrug resistance of Gram-negative bacteria) in 26 Burkholderia genomes in order to gain knowledge regarding their presence and distribution, to obtain a platform for future experimental tests aimed to identify new molecular targets to be used in antimicrobial therapy against Burkholderia species and to refine the annotation of RND-like sequences in these genomes.
Materials & Methods:
A total of 417 coding sequences were retrieved and analyzed using different bioinformatics tools.
Results & Conclusion:
A complex pattern of RND presence and distribution in the different Burkholderia species was disclosed and a core of proteins represented in all 26 genomes was identified. These 'core' proteins might represent useful targets of new synthetic antimicrobial compounds. Furthermore, the annotation of RND-like sequences in Burkholderia was refined.
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