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Methodology for the Study of Horizontal Gene Transfer in Staphylococcus aureus
Published on: March 10, 2017
Stability of Staphylococcus aureus phage ISP after freeze-drying (lyophilization)
Maia Merabishvili1, Chris Vervaet, Jean-Paul Pirnay
1Laboratory for Molecular and Cellular Technology, Queen Astrid Military Hospital, Brussels, Belgium. maia.merabishvili@mil.be
Abstract:
Staphylococcus aureus phage ISP was lyophilized, using an Amsco-Finn Aqua GT4 freeze dryer, in the presence of six different stabilizers at different concentrations. Stability of the lyophilized phage at 4 °C was monitored up to 37 months and compared to stability in Luria Bertani broth and physiological saline at 4 °C. Sucrose and trehalose were shown to be the best stabilizing additives, causing a decrease of only 1 log immediately after the lyophilization procedure and showing high stability during a 27 month storage period.
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