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Updated: May 9, 2026

Force Spectroscopy of Single Protein Molecules Using an Atomic Force Microscope
Published on: February 28, 2019
Polynomial force approximations and multifrequency atomic force microscopy
Daniel Platz1, Daniel Forchheimer, Erik A Tholén
1KTH Royal Institute of Technology, Section for Nanostructure Physics, Albanova University Center, SE-106 91 Stockholm, Sweden.
Abstract:
We present polynomial force reconstruction from experimental intermodulation atomic force microscopy (ImAFM) data. We study the tip-surface force during a slow surface approach and compare the results with amplitude-dependence force spectroscopy (ADFS). Based on polynomial force reconstruction we generate high-resolution surface-property maps of polymer blend samples. The polynomial method is described as a special example of a more general approximative force reconstruction, where the aim is to determine model parameters that best approximate the measured force spectrum. This approximative approach is not limited to spectral data, and we demonstrate how it can be adapted to a force quadrature picture.
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