Related Experiment Video
Updated: May 9, 2026

Methods to Study Epithelial Transport Protein Function and Expression in Native Intestine and Caco-2 Cells Grown in 3D
Published on: March 16, 2017
Transepithelial transports of rare sugar D-psicose in human intestine
Takashi Hishiike1, Masahiro Ogawa, Shigeru Hayakawa
1Department of Applied Biological Science, Faculty of Agriculture, Kagawa University, Kita-gun, Kagawa, Japan.
Abstract:
D-Psicose (Psi), the C3-epimer of D-fructose (Fru), is a noncalorie sugar with a lower glycemic response. The trans-cellular pathway of Psi in human enterocytes was investigated using a Caco-2 cell monolayer. The permeation rate of Psi across the monolayer was not affected by the addition of phlorizin, an inhibitor of sugar transporter SGLT1, whereas it was accelerated by treatment with forskolin, a GLUT5-gene inducer, clearly showing that GLUT5 is involved in the transport of Psi. The permeability of Psi was suppressed in the presence of D-glucose (Glc) and Fru, suggesting that the three monosaccharides are transported via the same transporter. Since GLUT2, the predominant sugar transporter on the basolateral membrane of enterocytes, mediates the transport of Glc and Fru, Psi might be mediated by GLUT2. The present study shows that Psi is incorporated from the intestinal lumen into enterocytes via GLUT5 and is released to the lamina propria via GLUT2.
More Related Videos
07:18Visualizing Monocarboxylates and Other Relevant Metabolites in the Ex Vivo Drosophila Larval Brain Using Genetically Encoded Sensors
Published on: October 27, 2023
09:13Two-dimensional Porcine Intestinal Organoids Reflecting the Physiological Properties of Native Gut
Published on: January 31, 2025
Related Concept Videos
Glucose Absorption Into the Small Intestine
Transcellular Transport of Solutes
Glucose Transporters
Facilitated diffusion-glucose transporters (GLUTs) are encoded by the solute-linked carrier (SLC) family 2, subfamily A gene family, or SLC2A. The 14 GLUT protein members are distributed into three classes:
Carbohydrate Absorption
After being swallowed, the partially digested carbohydrates mix with gastric secretions in the stomach. However, the acidic environment...
Secondary Active Transport
Secondary Active Transport