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Isolation, structure characterization and quantification of related impurities in asperosaponin VI
De Ji1, Yun-Fei Liu, Qiao-Han Wang
1State Key Laboratory of Natural Products and Functions, China Pharmaceutical University, Nanjing 210009, China.
Aim:
To study the related impurities in asperosaponin VI bulk drug and to develop a high performance liquid chromatography (HPLC) method for the determination of asperosaponin VI and its related impurities.
Methods:
The related impurities were detected in asperosaponin VI bulk drug by a newly developed HPLC method, obtained by ODS column chromatography and semi-preparative HPLC methods, and the structures were elucidated by TOF-MS, IR, and NMR techniques. The HPLC method was validated according to ICH guidelines for asperosaponin VI and its related impurities.
Results:
Seven related impurities (Imp 1-7) were isolated from asperosaponin VI bulk drug. Impurity 3 was found to be a mixture of two epimers, and was first reported in the paper. The validation results showed good sensitivity, specificity, linearity (r(2) ≥ 0.997 9), precision (RSD < 5.0%), accuracy (recoveries in the range of 94.61%-106.51%) and robustness.
Conclusion:
The developed HPLC method is suitable for the quality control of asperosaponin VI bulk drug.
