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Quantification of urinary 18-hydroxycortisol using LC-MS/MS.

Shigeki Jin1, Norio Wada, Yuji Takahashi

  • 1Faculty of Health Sciences, Hokkaido University, Sapporo, Japan.

Annals of Clinical Biochemistry
|July 13, 2013
PubMed
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A new liquid chromatography-tandem mass spectrometry method accurately measures urinary 18-hydroxycortisol. This validated assay aids in distinguishing aldosterone-producing adenoma (APA) from other conditions.

Area of Science:

  • Endocrinology
  • Analytical Chemistry
  • Mass Spectrometry

Background:

  • Urinary 18-hydroxycortisol is a potential biomarker for aldosterone-producing adenoma (APA).
  • Accurate measurement is crucial for diagnosing APA and differentiating it from other hypertensive disorders.

Purpose of the Study:

  • To develop and validate a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for quantifying urinary 18-hydroxycortisol.
  • To assess the method's utility in the diagnosis of APA.

Main Methods:

  • Urine samples were collected from patients with APA, idiopathic hyperaldosteronism (IHA), and essential hypertension (EH).
  • 18-Hydroxycortisol was extracted using solid-phase techniques.
  • Measurements were performed using LC-MS/MS with selected reaction monitoring.
Keywords:
Steroidaldosteronismhypertensionmass spectrometry

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Main Results:

  • The LC-MS/MS method demonstrated a low limit of quantification (0.26 nmol/L) and high precision (<3.4% CV).
  • Analytical recovery ranged from 98.0% to 103.7%.
  • Urinary 18-hydroxycortisol levels were significantly higher in APA patients (725 nmol/day) compared to IHA (102 nmol/day) and EH (88 nmol/day).

Conclusions:

  • The developed LC-MS/MS method is analytically robust and suitable for clinical application.
  • This validated assay can be a valuable tool for screening and differential diagnosis of APA.