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Updated: May 9, 2026

Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells
Published on: December 11, 2021
Integrated fluorescence correlation spectroscopy device for point-of-care clinical applications
Eben Olson1, Richard Torres, Michael J Levene
1Department of Biomedical Engineering, Yale School of Engineering and Applied Science, New Haven, CT 06510, USA.
Abstract:
We describe an optical system which reduces the cost and complexity of fluorescence correlation spectroscopy (FCS), intended to increase the suitability of the technique for clinical use. Integration of the focusing optics and sample chamber into a plastic component produces a design which is simple to align and operate. We validate the system by measurements on fluorescent dye, and compare the results to a commercial instrument. In addition, we demonstrate its application to measurements of concentration and multimerization of the clinically relevant protein von Willebrand factor (vWF) in human plasma.
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