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Updated: May 9, 2026

Morris Water Maze Test: Optimization for Mouse Strain and Testing Environment
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Published on: June 22, 2015

Dehydration parameters and standards for laboratory mice.

Christine M Bekkevold1, Kimberly L Robertson, Mary K Reinhard

  • 1Animal Care Services, University of Florida, Gainesville Florida, USA. cbekkevold@ufl.edu

Journal of the American Association for Laboratory Animal Science : JAALAS
|July 16, 2013
PubMed
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This summary is machine-generated.

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Acute water deprivation in mice causes significant physiological distress after 24 hours. Chronic water restriction is better tolerated, but prolonged deprivation is not humane for animal research.

Area of Science:

  • Animal Physiology
  • Laboratory Animal Science

Background:

  • Lack of data-driven humane standards for water deprivation/restriction in rodent studies.
  • Need for guidance for Institutional Animal Care and Use Committees (IACUCs), researchers, and veterinarians.

Purpose of the Study:

  • To establish humane endpoints for water deprivation/restriction in mice.
  • To investigate physiological and physical indicators of dehydration in mice.

Main Methods:

  • Acute water deprivation for up to 48 hours.
  • Chronic water restriction to 75% or 50% of ad libitum intake.
  • Measurement of physical and physiological dehydration indicators, including weight loss, plasma osmolality, plasma volume, and corticosterone levels.

Main Results:

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  • Acute deprivation >24 hours led to significant deterioration, >15% weight loss, increased plasma osmolality, decreased plasma volume, and elevated corticosterone.
  • No significant differences in dehydration measures were observed between conventional static cages and ventilated racks.
  • Chronic water restriction (up to 50% for 8 days) did not induce significant negative physiological changes.

Conclusions:

  • Acute water deprivation exceeding 24 hours is not recommended due to animal distress.
  • Chronic water restriction is adaptable for mice, but prolonged deprivation should be avoided.
  • Findings provide crucial data for humane animal research protocols involving water manipulation.