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In Vitro Differentiation Model of Human Normal Memory B Cells to Long-lived Plasma Cells
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Published on: January 20, 2019

Repression of the transcription factor Bach2 contributes to predisposition of IgG1 memory B cells toward plasma cell

Kohei Kometani1, Rinako Nakagawa, Ryo Shinnakasu

  • 1Laboratory for Lymphocyte Differentiation, Research Center for Allergy and Immunology, RIKEN, Yokohama, Kanagawa 230-0045, Japan.

Immunity
|July 16, 2013
PubMed

Memory B cells are essential for generating rapid and robust secondary antibody responses. It has been thought that the unique cytoplasmic domain of IgG causes the prompt activation of antigen-experienced IgG memory B cells. To assess this model, we have generated a mouse containing IgG1 B cells that have never encountered antigen. We found that, upon challenge, antigen-experienced IgG1 memory B cells rapidly differentiated into plasma cells, whereas nonexperienced IgG1 B cells did not, suggesting the importance of the stimulation history. In addition, our results suggest that repression of the Bach2 transcription factor, which results from antigen experience, contributes to predisposition of IgG1 memory B cells to differentiate into plasma cells.

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