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Related Concept Videos

Microbial Biosensors01:17

Microbial Biosensors

Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...
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Amperometry is a technique commonly used to measure the concentration of specific analytes in a solution by monitoring the electric current generated during an electrochemical reaction. It involves applying a constant potential between a working electrode and a reference electrode to measure the resulting current, which is proportional to the concentration of the analyte. The Clark oxygen electrode operates based on this principle of amperometry. It consists of a cathode and an anode enclosed...
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Automated diagnostic analyzers have transformed clinical microbiology by providing rapid and reliable methods for pathogen identification and antibiotic susceptibility testing. Among these systems, the Vitek 2 is widely used because it automates the traditionally labor-intensive processes of microbial identification (ID) and antibiotic susceptibility testing (AST), delivering standardized and timely results that are essential for effective patient care.Microbial Identification with ID CardsThe...

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Gold microwires based amperometric biosensor exploiting microbial architecture.

Shikha Sharma1, Sudha Srivastava

  • 1Department of Biotechnology, Jaypee Institute of Information Technology, Noida, UP 201307, India.

Biosensors & Bioelectronics
|July 16, 2013
PubMed
Summary
This summary is machine-generated.

Researchers created biocompatible gold microwires using fungal templates for biosensing. These novel nanomaterials offer high sensitivity and stability for detecting glucose and other applications.

Keywords:
BiocompatibleBiosensorGold microwiresMicrobial architecture

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Area of Science:

  • Nanotechnology and Biology Interface
  • Biomaterials Science
  • Electrochemistry

Background:

  • Controlled synthesis of nanomaterials is crucial for sensing and catalysis.
  • Microbial architectures offer unique templates for nanostructure fabrication.
  • Biocompatible nanomaterials are essential for biosensing applications.

Purpose of the Study:

  • To develop a novel method for synthesizing 1-D gold microwires using fungal hyphae as a template.
  • To evaluate the potential of these gold microwires as microelectrodes for biosensing.
  • To investigate the analytical performance characteristics of the fabricated gold microwires.

Main Methods:

  • Utilizing fungal hyphae as a scaffold for the assembly of amino acid functionalized gold nanoparticles (AuNPs).
  • Characterizing the resulting 1-D gold microwires for their morphology and properties.
  • Testing the gold microwires as microelectrodes for glucose detection via electron transfer.

Main Results:

  • Successfully synthesized biocompatible 1-D gold microwires with high sensitivity (43.2 µA/mM/cm²) and a wide linear range (5 µM to 20 mM) for glucose detection.
  • Demonstrated excellent repeatability (over 20 cycles) and reproducibility (RMSD as low as 1.3%) of the gold microwires.
  • Highlighted the role of electron delocalization, large surface area of AuNPs, and biocompatibility in achieving superior analytical performance.

Conclusions:

  • The fungal-templated gold microwires are promising microelectrodes for sensitive and stable biosensing.
  • This approach offers a versatile platform for creating functional nanomaterials for diverse applications.
  • The high biocompatibility and stability of the gold microwires suggest potential in various biosensing and catalytic domains.