Related Experiment Video
Updated: May 9, 2026

Expression and Purification of Nuclease-Free Oxygen Scavenger Protocatechuate 3,4-Dioxygenase
Published on: November 8, 2019
Functional characterization of PccH, a key cytochrome for electron transfer from electrodes to the bacterium
Joana M Dantas1, Diogo M Tomaz, Leonor Morgado
1Requimte-CQFB, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Campus Caparica, 2829-516 Caparica, Portugal.
Abstract:
The cytochrome PccH from Geobacter sulfurreducens (Gs) plays a crucial role in current-consuming fumarate-reducing biofilms. Deletion of pccH gene inhibited completely electron transfer from electrodes toward Gs cells. The pccH gene was cloned and the protein heterologously expressed in Escherichia coli. Complementary biophysical techniques including CD, UV-visible and NMR spectroscopy were used to characterize PccH. This cytochrome contains one low-spin c-type heme with His-Met axial coordination and unusual low-reduction potential. This reduction potential is pH-dependent, within the Gs physiological pH range, and is discussed within the context of the electron transfer mechanisms from electrodes to Gs cells.
More Related Videos
Related Concept Videos
Anoxygenic Photosynthesis
Electron Transport Chain: Complex III and IV
Anoxygenic Phototrophic Bacteria
The Photochemical Reaction Center
Electron Transport Chain Components
Microbial Nutrition

