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Related Experiment Video

Updated: May 9, 2026

Electrospun Nanofiber Scaffolds with Gradations in Fiber Organization
09:32

Electrospun Nanofiber Scaffolds with Gradations in Fiber Organization

Published on: April 19, 2015

Shape-memory-actuated change in scaffold fiber alignment directs stem cell morphology.

Ling-Fang Tseng1, Patrick T Mather, James H Henderson

  • 1Department of Biomedical and Chemical Engineering, 121 Link Hall, Syracuse University, Syracuse, NY 13244, USA; Syracuse Biomaterials Institute, 318 Bowne Hall, Syracuse University, Syracuse, NY 13244, USA.

Acta Biomaterialia
|July 16, 2013
PubMed
Summary

This study introduces a novel thermoresponsive scaffold for tissue engineering that dynamically changes shape and fiber alignment. This dynamic scaffold successfully controlled cell behavior and alignment during culture, demonstrating potential for advanced mechanobiology applications.

Keywords:
Cell cultureScaffoldShape memoryThermally responsive material

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Patterning the Geometry of Human Embryonic Stem Cell Colonies on Compliant Substrates to Control Tissue-Level Mechanics
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Patterning the Geometry of Human Embryonic Stem Cell Colonies on Compliant Substrates to Control Tissue-Level Mechanics

Published on: September 28, 2019

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Last Updated: May 9, 2026

Electrospun Nanofiber Scaffolds with Gradations in Fiber Organization
09:32

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Published on: April 19, 2015

Patterning the Geometry of Human Embryonic Stem Cell Colonies on Compliant Substrates to Control Tissue-Level Mechanics
10:04

Patterning the Geometry of Human Embryonic Stem Cell Colonies on Compliant Substrates to Control Tissue-Level Mechanics

Published on: September 28, 2019

Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Mechanobiology

Background:

  • Traditional tissue engineering scaffolds are static and fail to mimic dynamic in vivo microenvironments.
  • Dynamic control over scaffold architecture is crucial for guiding cell behavior.

Purpose of the Study:

  • To develop and evaluate a thermoresponsive, shape-memory polymer (SMP) scaffold capable of dynamic architectural changes.
  • To investigate if shape-memory-actuated changes in scaffold fiber alignment can control the behavior of attached and viable cells.

Main Methods:

  • Electrospinning of a shape memory polymer (SMP) to create a thermoresponsive scaffold.
  • Straining the scaffold to align fibers, followed by cell seeding and culture (human adipose-derived stem cells).
  • Utilizing a cytocompatible temperature increase to trigger shape memory actuation, reverting the scaffold to its original shape and random fiber orientation.

Main Results:

  • Cells preferentially aligned along the strain-induced fiber direction on the scaffold before shape memory actuation.
  • After shape memory actuation, cells remained attached and viable but lost their preferential alignment.
  • Demonstrated that shape-memory-actuated changes in scaffold fiber alignment can control cell morphological behavior.

Conclusions:

  • Shape memory actuation in cytocompatible scaffolds can dynamically alter fiber alignment and influence cell behavior.
  • This technology holds promise for advancing tissue engineering scaffold development, delivery, and functionality.
  • Facilitates in vitro and in vivo studies of mechanobiology by providing dynamic microenvironmental cues.