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Updated: May 9, 2026

A Protein Microarray Assay for Serological Determination of Antigen-specific Antibody Responses Following Clostridium difficile Infection
Published on: June 15, 2018
Potent monoclonal antibodies against Clostridium difficile toxin A elicited by DNA immunization
Chunhua Zhang1, Ke Jin1, Yanling Xiao2
1Jiangsu Province Key Laboratory in Infectious Diseases; The First Affiliated Hospital of Nanjing Medical University; Nanjing, P.R. China; Department of Infectious Diseases; The First Affiliated Hospital of Nanjing Medical University; Nanjing, P.R. China; China-US Vaccine Research Center; The First Affiliated Hospital of Nanjing Medical University; Nanjing, P.R. China.
Abstract:
Recent studies have demonstrated that DNA immunization is effective in eliciting antigen-specific antibody responses against a wide range of infectious disease targets. The polyclonal antibodies elicited by DNA vaccination exhibit high sensitivity to conformational epitopes and high avidity. However, there have been limited reports in literature on the production of monoclonal antibodies (mAb) by DNA immunization. Here, by using Clostridium difficile (C. diff) toxin A as a model antigen, we demonstrated that DNA immunization was effective in producing a panel of mAb that are protective against toxin A challenge and can also be used as sensitive reagents to detect toxin A from various testing samples. The immunoglobulin (Ig) gene usage for such mAb was also investigated. Further studies should be conducted to fully establish DNA immunization as a unique platform to produce mAb in various hosts.
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