Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Ultra-high performance, solid-state, autoradiographic image digitization and analysis system.

J L Lear1, J P Pratt, R F Ackermann

  • 1Department of Radiology, University of Colorado Health Sciences Center, Denver 80262.

Metabolic Brain Disease
|June 1, 1990
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Ultrasound-guided internal jugular vein catheterization: a randomized controlled trial.

Heart, lung and vessels·2014
Same author

Endocrine disorders of pregnancy.

Bulletin. Chicago Medical Society·2010
Same author

Psychosomatic gynecology.

Journal of the Medical Association of Georgia·2010
Same author

Composition of the human placenta; proximate composition.

American journal of obstetrics and gynecology·2010
Same author

Composition of the human placenta; lipid content.

American journal of obstetrics and gynecology·2010
Same author

The stimulation and maintenance of lactation.

The American journal of nursing·2010
Same journal

The protective role of melatonin on the brain in a rat model of Alzheimer's disease.

Metabolic brain disease·2026
Same journal

Neuroprotective effects of 2-(2-nitrophenyl)-1,3-thiazolidine-4-carboxylic acid in a rotenone-induced Parkinson's disease rat model: Modulation of oxidative stress, behavioral dysfunction, and iron accumulation.

Metabolic brain disease·2026
Same journal

Gestational deltamethrin exposure exacerbates post-traumatic epileptiform activity and social deficits, diminishing the therapeutic efficacy of curcumin in rats.

Metabolic brain disease·2026
Same journal

The interplay between gut microbiota and Alzheimer's disease: mechanistic insights from dysbiosis to disease modulation.

Metabolic brain disease·2026
Same journal

Bushen Huoxue Acupuncture alleviates alzheimer's disease progression via the E3 ubiquitin ligase SMURF2‑mediated ubiquitination of LAPTM5.

Metabolic brain disease·2026
Same journal

Gut-brain axis modulation by fecal microbiota transplantation improves dual-organ injury after cerebral ischemia-reperfusion via Caspase-8 dependent inhibition of necroptosis.

Metabolic brain disease·2026
See all related articles

A new charge-coupled device (CCD) scanner and Macintosh II computer system digitizes autoradiographic images rapidly and accurately. This high-resolution system offers superior performance over existing video camera and rotating drum densitometer methods.

Area of Science:

  • Biomedical Imaging
  • Image Analysis
  • Scientific Instrumentation

Background:

  • Autoradiography is crucial for quantifying biological processes.
  • Existing digitization systems have limitations in speed, resolution, or accuracy.
  • High-speed, high-resolution quantification of autoradiographic data is needed.

Purpose of the Study:

  • To develop and evaluate a novel Macintosh II-based system for high-speed, high-resolution autoradiographic image digitization and analysis.
  • To assess the accuracy and speed of the developed system compared to existing technologies.

Main Methods:

  • A linear charge-coupled device (CCD) array was integrated with a precision drive assembly and Macintosh II computer.
  • The system digitized 20 x 25-cm autoradiographic films at 50-micron resolution and 256 gray levels in under 30 seconds.

Related Experiment Videos

  • Software was developed for data analysis, and system accuracy was evaluated by repeated scanning and variability measurements.

    • Main Results:

    • The developed CCD scanner-Macintosh system achieved virtually noise-free image data.
    • The coefficient of variation averaged less than 1%, surpassing video camera and rotating drum densitometer systems.
    • The system demonstrated significantly greater speed and accuracy than existing RDD scanners.

    Conclusions:

    • The CCD scanner-Macintosh computer system provides a significant advancement for autoradiographic data analysis.
    • This system offers superior speed and accuracy for digitizing entire films with multiple autoradiograms.
    • It represents a valuable tool for researchers requiring precise quantification of autoradiographic images.