Related Experiment Video
Updated: May 9, 2026
Isolation of Tonsillar Mononuclear Cells to Study Ex Vivo Innate Immune Responses in a Human Mucosal Lymphoid Tissue
Published on: June 14, 2020
CD64 expression on monocytes in children with adenoid hypertrophy
Fabio Pagella1, Mara De Amici, Elina Matti
1Fondazione IRCCS Policlinico San Matteo, Pavia, Italy. gio.cip@libero.it
Insights
Children with severe obstructive hypertrophy (AH) show increased CD64 expression on monocytes, indicating heightened immune activation. This finding may help identify severe cases of AH in pediatric patients.
Area of Science:
- Pediatric immunology
- Inflammatory markers
Background:
- Obstructive hypertrophy of adenoids (AH) is a common pediatric condition.
- Immune system activation is implicated in AH pathogenesis.
Purpose of the Study:
- To investigate the expression of CD64 on monocytes in children with AH.
- To explore the association between CD64 expression and the severity of AH.
Main Methods:
- Monocyte CD64 expression was measured in 66 children with AH undergoing adenoidectomy or adeno-tonsillectomy.
- A control group of 30 healthy children was included for comparison.
Main Results:
- Children with severe AH exhibited significantly higher CD64 expression on monocytes compared to those with less severe AH (size 3) and healthy controls.
- Elevated CD64 levels suggest increased monocyte activation in severe AH cases.
Conclusions:
- CD64 expression on monocytes may serve as a potential biomarker for severe obstructive hypertrophy in children.
- This preliminary finding suggests a more pronounced immune response in children with severe AH.
Objective:
Adenoids with obstructive hypertrophy (AH) are considered to be one of the most ancient and common problems in Paediatrics. This study aimed at investigating the expression of CD 64 on monocytes in children who had to undergo adenoidectomy.
Methods:
Therefore, 66 children (24 females, 42 males, mean age 4.9 years), affected by AH and requiring adenoidectomy (54%) or adeno-tonsillectomy (46%) were consecutively enrolled into the study. Moreover, 30 healthy children (11 females, 19 males, mean age 11.2 years) were enrolled in this study as controls. CD64 expression on monocytes was measured.
Results:
Children with severe AH had higher CD64 values than children with AH size 3 and controls.
Conclusions:
This preliminary study shows that CD64 expression on monocytes may be associated with severe AH, suggesting a more intense immune activation in these subjects.
More Related Videos
08:31Characterization of Human Monocyte-derived Dendritic Cells by Imaging Flow Cytometry: A Comparison between Two Monocyte Isolation Protocols
Published on: October 18, 2016
09:57Flow Cytometric Analysis for Identification of the Innate and Adaptive Immune Cells of Murine Lung
Published on: November 16, 2021